Objective To investigate the expression of PD1 on CD4 + T and CD8 + T from the patients with rheumatoid arthritis (RA) and to analyze the clinical relevance to disease severity.Methods The expression of PD1 on CD4 + T and CD8 + T were examined from 66 RA patients and 30 healthy controls (HC) by the technique of flow cytometry.The expression of PD1 including mean fluorescence intensity (MFI) on CD4 + T and CD8 + T and percentage of CD4 + PD1 + T and CD8 + PD1 + T were compared between RA patients and HC.Moreover,its correlation with laboratory inspection was analyzed.Results ① The MFI of PD1 on CD4 + T and CD8 + T from RA patients significantly elevated compared with HC (P ＜ 0.05),and the percentage of CD4 + PD1 + T from RA patients significantly elevated compared with HC (P ＜0.05).② The MFI of PD1 on CD4+T and CD8+ T in RA patients were positively associated with DAS28 (r =0.49,P ＜0.000 1;rs =0.40,P =0.000 8).③ The MFI of PD1 on CD4 +T in RA patients were positively associated with CRP and ESR (r =0.45,P ＜ 0.000 1;r =0.39,P =0.001).The MFI of PD1 on CD8 +T in RA patients was positively associated with CRP(rs =0.40,P =0.000 9).The percentage of CD4 + PD1 + T in RA patients was positively associated with CRP (r =0.24,P =0.048).④ The MFI of PD1on CD4 + T in RA patients were positively associated with RF (rs =0.32,P =0.009).The percentage of CD4 +PD1 + T in RA patients was positively associated with RF(r =0.27,P =0.030).Conclusion The aberrations ex-pression of PD1 on CD4 + T and CD8 + T are observed in patients with RA.Increased expression of PD1 on CD4 + Tand CD8 + T are correlated with disease activity,and also the production of antibody.%目的 探讨程序性死亡分子1(PD1)在类风湿关节炎(RA)患者外周血CD4+T和CD8+T细胞上的表达及临床意义.方法 应用流式细胞仪检测66例RA患者和30例健康对照者外周血CD4+T和CD8+T细胞上PD1表达(平均荧光强度和百分率),比较RA组和健康对照组之间CD4+T和CD8+T细胞上PD1表达水平,分析其与RA实验室检查指标的相关性.结果 ①RA患者CD4+T、CD8+T细胞PD1表达的平均荧光强度(MFI)显著高于对照组,差异均有统计学意义(P＜0.05);RA患者CD4+ PD1+T细胞百分率显著高于对照组,差异有统计学意义(P＜0.05).②RA患者外周血CD4+T、CD8+T细胞PD1表达的MFI与DAS28评分呈正相关性(r =0.49,P＜0.000 1;r =0.40,P=0.0008).③RA患者外周血CD4+T细胞PD1表达的MFI与血沉(ESR)、C-反应蛋白(CRP)呈正相关性(r=0.45,P＜0.000 1;r=0.39,P=0.001);RA患者外周血CD8+T细胞PD1表达的MFI与CRP呈正相关性(rx=0.40,P=0.0009);RA患者外周血CD4+ PD1+T细胞百分率与CRP呈正相关性(r =0.24,P=0.048).④RA患者外周血CD8+T细胞PD1表达的MFI与类风湿因子(RF)呈正相关性(rs=0.32,P =0.009);RA患者外周血CD4+ PD1+T细胞百分率与RF呈正相关性(r=0.27,P=0.030).结论 RA患者外周血CD4+T和CD8+T细胞PD1表达异常,与疾病的活动性及抗体产生有关.
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