目的 体外探讨聚桂醇对人肝癌HepG2细胞的生长、迁移影响及血管内皮生长因子(VEGF)的表达调控.方法 应用MTS比色法观察不同浓度的聚桂醇对人肝癌细胞系细胞的生长抑制作用,并观察细胞在各时间点的抑制率;平板克隆实验检测细胞克隆形成能力的变化;用细胞划痕实验观察对肝癌细胞迁移的影响;Western blot检测VEGF蛋白的表达变化.结果 聚桂醇以剂量依赖的方式抑制HepG2细胞生长,0、20、40、60及80 μg/ml聚桂醇对肝癌细胞的生长抑制率分别为0、26.78%、35.37%、51.52%和67.15%(P<0.05);聚桂醇能抑制肝癌细胞的克隆形成、迁移及VEGF蛋白表达,且抑制VEGF蛋白表达跟作用时间呈正相关.结论 聚桂醇能抑制肝癌细胞增殖及迁移,影响VEGF 蛋白表达.%Objective To explore the effects of Lauromacrogol on the cell growth , migration and the expression of vascular endothelial growth factor ( VEGF ) in HepG2 cells in vitro. Methods The inhibition effects during proliferation of HepG2 cells treated with Lauromacrogol were evaluated with MTS assay; cloning formation assay and scratching assay were used to study the effects on proliferation and mobility capability of liver cancer cells; the expression of VEGF was detected with Western blot. Results Lauromacrogol inhibited the growth of HepG2 cells in a dose-dependent manner. The growth inhibitory rates of HepG2 cells treated with 0, 20, 40, 60, and 80 μg/ml Lauromacrogol were 0% ,26. 78% ,35. 37% ,51. 52% and 67. 15% , respectively ( P <0. 01 ). Lauromacrogol significantly inhibited the growth, migration and down-regulated the expression of VEGF with time-dependance. Conclusion Lauromacrogol inhibits liver cancer cell proliferation, migration and inhibits the expression of VEGF.
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