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首页> 外文期刊>Journal of prosthetics and orthotics: JPO >Effect of total saponins from marsdenia tenacissima on the proliferative inhibition of human liver cancer HepG2 cells
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Effect of total saponins from marsdenia tenacissima on the proliferative inhibition of human liver cancer HepG2 cells

机译：总皂苷的影响来自Marsdenia Tenacissima对人肝癌HepG2细胞增殖抑制作用

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? 2019, Chinese Medical Association Publishing House Co. Ltd. All rights reserved. ? 2019, Chinese Medical Association Publishing House Co. Ltd. All rights reserved. Objective To investigate the proliferative inhibition effect and mechanism of total saponins from marsdenia tenacissima on human liver cancer HepG2 cells. Methods Human liver cancer HpeG2 cells cultured conventionally were divided into the marsdenia tenacissima saponins A control group, the experimental group and the blank control group. The experimental group was treated with different mass concentrations of total saponins from marsdenia tenacissima (0.14, 0.29, 0.58, 1.15, 2.13 mg/ml), while the marsdenia tenacissima saponins A control group was treated with marsdenia tenacissima saponins A (1.0 mg/ml), and the blank control group was established stimultaneously. The methyl thiazolyl tetrazolium (MTT) method was used to detect the effect of total saponins from marsdenia tenacissima on the activity of HepG2 cells, and the cell morphology was observed by using inverted microscopy. The cell apoptosis rate was detected by using flow cytometry from Annexin V-FITC/PI staining. The expressions of bcl-2, bax and p53 were analyzed by using Western blot after the drug effect. Results The results of cell activity test showed that total saponins from marsdenia tenacissima could inhibit the proliferation of HepG2 cells at the concentration of 0.29, 0.58, 1.15, 2.13 mg/ml, which was positively correlated with the concentration. The half maximal inhibitory concentration (IC 50) of cell growth inhibition of total saponins from marsdenia tenacissima on HepG2 cells was 0.75 mg/ml. Under inverted microscopy, the adherent cells were significantly reduced, the cells fell off into clusters and the debris was increased after the effect of total saponins from marsdenia tenacissima. Moreover, flow cytometry showed that total saponins from marsdenia tenacissima could increase the late apoptosis rate of HepG2 cells (P 0.01). Western blot showed that the expression of bcl-2 was 0.62±0.16, 0.31 ±0.15, 0.84±0.09 and 1.00±0.11 respectively in the experimental group (total saponins from marsdenia tenacissima 0.58 mg/ml and 2.13 mg/ml), the marsdenia tenacissima saponins A control group and the blank control group; the expression of bax protein was 0.75±0.10, 0.83±0.12, 1.00±0.14 and 0.15±0.02, respectively in the above four groups; the expression of p53 protein was 0.63 ±0.08, 0.78±0.11, 1.00±0.13 and 0.18±0.02 respectively in the above four groups. The protein expression of bcl-2 was decreased and the protein expressions of bax and p53 were increased in the experiment group, and there was a statistical difference between the experiment group and the blank control group (P 0.05). Conclusions Total saponins from marsdenia tenacissima can upregulate the expression of bax by upregulating the expression of p53 gene, and inhibit the expression of bcl-2, which would cause the cascade reaction to induce the apoptosis of HepG2 cells. Its inhibitory effect can be realized through mitochondrial pathmay to induce apoptosis. Objective To investigate the proliferative inhibition effect and mechanism of total saponins from marsdenia tenacissima on human liver cancer HepG2 cells. Methods Human liver cancer HpeG2 cells cultured conventionally were divided into the marsdenia tenacissima saponins A control group, the experimental group and the blank control group. The experimental group was treated with different mass concentrations of total saponins from marsdenia tenacissima (0.14, 0.29, 0.58, 1.15, 2.13 mg/ml), while the marsdenia tenacissima saponins A control group was treated with marsdenia tenacissima saponins A (1.0 mg/ml), and the blank control group was established stimultaneously. The methyl thiazolyl tetrazolium (MTT) method was used to detect the effect of total saponins from marsdenia tenacissima on the activity of HepG2 cells, and the cell morphology was observed by using inverted
机译：还2019年，中国医学协会出版社有限公司保留所有权利。还2019年，中国医学协会出版社有限公司保留所有权利。目的探讨Marsdenia Tenacissima对人肝癌HepG2细胞的增殖抑制作用及机制。方法培养的人肝癌HPEG2细胞常规培养为MARSDENIA TENACISSIMA SAPONINS对照组，实验组和空白对照组。实验组用来自Marsdenia Tenacissima的不同皂苷的实验组处理（0.14,0.29,0.58,1.15,2.13mg / ml），而Marsdenia Tenacissima saponins是用Marsdenia Tenacissima Saponins A治疗的玛登西亚·Tenacissima Saponins（1.0mg / ml ），空白对照组被显着建立。使用甲基噻唑基四唑（MTT）方法检测总皂苷从MARSDENIA TENACISSIMA对HepG2细胞活性的影响，并且通过使用倒置显微镜观察细胞形态。通过使用来自膜蛋白V-FITC / PI染色的流式细胞术检测细胞凋亡率。通过在药物效果后使用蛋白质印迹分析Bcl-2，Bax和P53的表达。结果细胞活性试验结果表明，MARSDENIA TENACISSIMA的总皂苷可以抑制浓度为0.29,0.58,1.15,2.13mg / ml的HepG2细胞的增殖，其与浓度正相关。来自Marsdeia Tenacissima的细胞生长抑制的半最大抑制浓度（IC 50）HepG2细胞中的皂苷为0.75mg / ml。在倒置显微镜下，粘附细胞显着降低，细胞落后于簇状物，在总皂苷来自Marsdenia tenacissima的效果后，碎片增加。此外，流式细胞术显示来自Marsdenia Tenacissima的总皂苷可以增加HepG2细胞的晚期凋亡率（P <0.01）。 Western印迹表明，在实验组中，Bcl-2的表达分别为0.62±0.16,0.61±0.15,0.84±0.09和1.00±0.11（来自Marsdenia Tenacissima 0.58 mg / ml和2.13mg / ml）的总皂苷Tenacissima Saponins一个对照组和空白对照组; Bax蛋白表达为0.75±0.10，0.83±0.12，1.00±0.14和0.15±0.02，分别在上述四组;在上述四组中，P53蛋白的表达分别为0.63±0.08,0.78±0.11,1.00±0.13和0.18±0.18±0.18±0.18.18±0.02。降低Bcl-2的蛋白质表达，实验组中，Bax和P53的蛋白质表达增加，实验组和空白对照组之间存在统计学差异（P <0.05）。结论Marsdenia Tenacissima的总皂苷通过上调P53基因的表达，抑制Bcl-2的表达，这将导致级联反应诱导HepG2细胞的凋亡。通过线粒体途径来实现其抑制作用以诱导细胞凋亡。目的探讨Marsdenia Tenacissima对人肝癌HepG2细胞的增殖抑制作用及机制。方法培养的人肝癌HPEG2细胞常规培养为MARSDENIA TENACISSIMA SAPONINS对照组，实验组和空白对照组。实验组用来自Marsdenia Tenacissima的不同皂苷的实验组处理（0.14,0.29,0.58,1.15,2.13mg / ml），而Marsdenia Tenacissima saponins是用Marsdenia Tenacissima Saponins A治疗的玛登西亚·Tenacissima Saponins（1.0mg / ml ），空白对照组被显着建立。使用甲基噻唑酯四唑（MTT）方法检测总皂苷从MARSDENIA TENACISSIMA对HepG2细胞活性的影响，并且通过倒置观察细胞形态

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