Objective To study the changes and significance of KISS-1 mRNA and β-HCG mRNA in maternal plasma with preeclampsia. Methods Peripheral blood samples were obtained from 40 pregnant women with severe preeclampsia, 35 pregnant women with mild preeclampsia and 30 normal pregnant women. RNA was extracted and levels of KISS-1 mRNA and β-HCG mRNA were determined by real-time quantitative PCR. Results All pregnant women were found expression of KISS-1 mRNA and β-HCG mRNA. mRNA levels of KISS-1 and β-HCG were increased in plasma from pregnant women with severe preeclampsia compared with those from pregnant women with mild preeclampsia( P < 0. 01 ), but also significantly increased compared with those from normal pregnant women( P < 0. 01 ). Conclusion KISS-1 and β-HCG mRNA are distributed differently in pregnant women with or without preeclampsia, which are higher in pregnant women with preeclampsia, and may be used as reference of diagnosis of preeclampsia.%目的 研究子痫前期患者母血中胎儿游离肿瘤转移抑制因子(KISS-1)mRNA和人绒毛膜促性腺激素β亚单位(β-HCG)mRNA的表达变化及意义.方法 选择重度子痫前期患者40例,轻度子痫前期患者35例,对照组正常孕妇30例,提取其外周血血浆中游离mRNA,通过实时荧光定量PCR检测KISS-1 mRNA和β-HCG mRNA的表达.结果 各组样本中均可检测到KISS-1 mRNA和β-HCG mRNA表达.其中KISS-1 mRNA和β-HCG mRNA表达量在重度子痫前期组明显高于轻度子痫前期、对照组(P<0.01).轻度子痫前期组KISS-1 mRNA和β-HCG mRNA表达量高于对照组(P<0.01).结论 孕妇血浆中胎盘来源的游离KISS-1 mRNA和β-HCG mRNA的表达对子痫前期发病具有预测价值.
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