目的 探讨饮食、运动对高脂饲料喂养的肥胖大鼠肌肉组织中细胞内转接蛋白(APPL1)、葡萄糖转运蛋白4(GLUT4)的影响,以及APPL1与GLUT4的关系.方法 70只4周龄SD大鼠随机分为5组:正常对照组(NC组)、高脂肥胖组(HF组)、饮食控制组(DC组)、运动锻炼组(EC组)、饮食运动结合组(DE组).NC组给予普通饮食,其余各组给予高脂饮食.12周后,DC组饮食限制,EC组进行游泳运动,DE组两者结合,共 6周,空腹取血检测相关代谢指标,取骨骼肌组织检测甘油三脂(TG)的含量和APPL1、GLUT4的表达.结果 HF组大鼠体重、TG、总胆固醇(TCH)、空腹血糖(FBG)、空腹胰岛素(FINS)明显高于NC组(P<0.05),APPL1、GLUT4的水平明显低于NC组(P<0.05),骨骼肌组织中的TG水平明显增加(P<0.05).饮食、运动干预后能减轻体重(P<0.05),降低TG、TCH水平(P<0.05),增加胰岛素敏感指数(P<0.05),降低骨骼肌组织中的TG水平(P<0.05),上调APPL1、GLUT4的表达(P<0.05).结论 高脂饮食可诱导大鼠的胰岛素抵抗模型.通过饮食控制和(或)运动锻炼均可降低高脂喂养的脂质水平,改善胰岛素抵抗,上调骨骼肌组织中APPL1、GLUT4水平.%Objective To investigate the effects of diet control and exercise on APPL1, glucose transporter 4 ( GLUT4 ) and their correlation in high fat diet induced obese rats. Methods 70 SD rats of 4 weeks, age were randomly divided into five groups: normal control group ( NC group ), high-fat diet group( HF group ), diet control group ( DC group ),exercise control group( EC group )and diet and exercise control group( DE group ). The animals of NC group were fed with normal chow and the others with high-fat diet. After 12 weeks, the rats of DC group were given diet control for 6 weeks, at the same time, EC group swam everyday, DE group had to do both two interventions. Then the blood of rats in fasting was obtained to measure metabolic indexs, triglyceride lipid and expression of AP-PL1, GLUT4 were measured in skeletal muscle tissues. Results Body weight, triglyceride( TG ), total cholesterol ( TCH ), fasting blood glucose( FBG ), fasting insulin( FINS ) and the TG level in skeletal muscle tissue of HF group were significantly higher than those of NC group( P < 0. 05 )expression of APPL1, GLUT4 of HF group were significantly lower. Diet, exercise intervention could reduce body weight( P < 0. 05 ), decrease TG, TCH( P < 0. 05 ), increase insulin sensitivity index( P < 0. 05 ), decrease glycerin level in skeletal muscle tissue( P < 0. 05 ), and enhance the expression of APPL1, GLUT4( P < 0. 05 ). Conclusion High fat diet could induce the model of insulin resistance. Diet control and/or exercise can reduce lipid levels of high-fat diet, improve insulin resistance and up-regulate expressions of APPL1, GLUT4 in skeletal muscle tissue.
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