The purities of cephalomannine and taxol in their crude extracts were 33.1% and 36.5%, respectively,determined by high performance liquid chromatography(HPLC).Then the above cephalo-mannine and taxol were further separated by high speed countercurrent chromatography with n-hexane-methanol-ethanol-water-acetic acid ethyl ester(5: 7: 5:1:6.5,vd /v)mobile phase,and their pu-rity were 88.4% and 89.7%,respectively. Finally,the purified cephalomannine and taxol were recrystal-lized with 50% acetonitrile and their purities were up to 98.7% and 97.6%,respectively.%通过高效液相色谱测定紫杉醇粗提物中三尖杉宁碱和紫杉醇的纯度分别为33.1% 和36.5%,采用正己烷-醋酸乙酯-甲醇-乙醇-水(5:7:5:1:6.5,v/v)体系,利用高速逆流色谱仪分离得到三尖杉宁碱和紫杉醇馏出物纯度分别为88.4% 和89.7%,以50% 乙腈进行重结晶后,三尖杉宁碱和紫杉醇纯度分别为98.7% 和97.6%.
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