Objective To explore the epidemiological characteristic of patients with hand, foot and mouth diseases ( HFMD) hospi-talized in hospital of Suixi county in Anhui province from Jan 2010 to Dec 2013, so as to provide scientific basis for prevention and control of hand, foot and mouth disease. Methods Real-time fluorescence quantitative RT-PCR kits with three reagents: universal enterovirus primer, coxsackievirus A16 (CoxA16) primers and enterovirus 71 (EV71) primers were used to detect throat swab samples, respectively. Results Of pharyngeal swab specimens in 251 HFMD patients, the enterovirus-positive rate was 28. 69% (72/251). Among this, the positive rate of EV71, CoxA16 and other types of entervirus was 19. 92% (50/251), 6. 77% (17/251), 1. 99% (5/251), respectively. In 72 enterovirus-positive patients, the positive rate of EV71, CoxA16 was 69. 44%, 23. 61%, respectively. CoxA16 had statistical signifi-cance in annual positive detection rate (P<0. 05). During the epidemic of HFMD in 2010-2013, EV71 was always in higher levels, as the predominant virus strains. The main peak of the epidemic Of HFMD was annually 3 -6 month, and most cases showed symptoms in 1 -3years old. The positive rate of enterovirus detection in gender, age, the epidemic seasons showed no statistical significance (P>0. 05) . Conclusion In HFMD epidemic-period of 2010-2013, pathogens of HFMD are mainly EV71 and CoxA16, dominated by EV71. The main peak of the epidemic Of HFMD is in annual 3-6 months, 1 to 3 years old infants, and young children should be strengthened for pre-vention and monitoring of foot and mouth disease.%目的：了解本地区手足口病( HFMD)病原及流行病学特征,为HFMD防治提供科学依据。方法251例临床诊断手足口病患者来自濉溪县医院2010年1月至2013年12月住院病例,采集咽拭子标本,应用实时荧光RT-PCR法检测标本中的人肠道病毒(HEV)、肠道病毒71型(EV71)和柯萨奇病毒A组16型(CoxA16)特异性核酸,分析手足口病的流行病学特征。结果251例HFMD患者标本,肠道病毒阳性率为28.69%(72/251),EV71阳性率为19.92%(50/251),CoxA16阳性率为6.77%(17/251),其他HEV阳性率为1.99%(5/251)。72例检测阳性病例中,EV71阳性病例50例占69.44%,CoxA1617例占23.61%,Cox-A16在年检测阳性率方面有统计学意义(P<0.05),EV71病毒在每年病原检测比例中都处于较高水平,为2010~2013年手足口病的优势病原。流行高峰主要是每年的3~6月份,发病年龄主要集中在1~3岁幼儿,肠道病毒检测阳性率在性别、年龄、流行季节方面比较无差异统计学意义(P>0.05)。结论2010~2013年手足口病流行中,病原体主要为以EV71和CoxA16,其中EV71是优势病毒型,应加强1~3岁婴幼儿手足口病的预防和监测。
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机译：在意大利的流行病学e monitoraggio alcol-correlato。 Valutazione dell'Osservatorio Nazionale alcol-CNEsps sull'impatto dell'uso e abuso di alcol ai fini dell'implementazione delle attivita del piano Nazionale alcol e salute。 2010年报告。（意大利的流行病学和酒精相关监测。国家酒精评估 - CNEsps关于使用和滥用酒精对支持实施国家酒精和健康计划活动的影响。2010年报告）
