Objective To study the protective effects of rutin on gastric mucosal lesions induced by ischemia/reperfusion (I /R)in rats and its underlying mechanisms.Methods SD rats were randomly divided into 5 groups.Under anesthesia,the celiac artery was clamped for 30 min,and then reperfusion was established by removal of the clamp for 60 min.After the reperfusion,the total area of ero-sions,the levels of nitrite itrate,the levels of gastric wall mucus and the activity of superoxide dismutase (SOD),myeloperoxidase (MPO),total NO synthase (TNOS),inducible NOS (iNOS),and constitutive NOS (cNOS)in gastric mucosa were examined.Moreo-ver,histological analysis of the lesions was also carried out.Following I /R,hemorrhagic lesions were generated in the mucosa.Results Prior administration of rutin before ischemia significantly ameliorated the gastric damage induced by I /R in a dose -dependent manner. It significantly reduced the index of neutrophil infiltration,lipid peroxidation and nitrite itrate content.Additionally,the decrease of gastric wall mucus and cNOS activity,the increases in activities of total NOS and iNOS in the gastric mucosa were also significantly at-tenuated by rutin.Conclusions These results indicated that rutin had a protective effect against I /R -induced gastric mucosal lesions in rats at least partly by preserving gastric wall mucus and secretion and reducing polymorphonuclear leukocyte infiltration into the mu-cosa via regulating the production of nitric oxide (NO).%目的：研究芦丁对胃缺血再灌注损伤的保护作用及其机制。方法采用大鼠胃缺血再灌注损伤模型，观察胃黏膜细胞形态学变化；测定胃壁黏液含量、胃黏膜组织中一氧化氮（NO）含量、丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性、髓过氧化物氧化酶（MPO）活性、总 NOS （TNOS）、诱导型（iNOS）和结构型 NOS （cNOS）活性。结果芦丁能显著降低胃黏膜损伤指数，维持胃黏膜缺血再灌注期 SOD 活力，降低 TNOS 和 iNOS 活性，上调 cNOS 活性，减少 MDA 含量，降低 MPO 活性，增加胃壁黏液分泌量，减轻缺血再灌注后胃黏膜病理损伤。结论芦丁对缺血再灌注引起的胃黏膜损伤具有保护作用，其作用机制可能与调节 NOS 活性，抑制淋巴细胞在胃黏膜组织中浸润，清除和抑制胃缺血再灌注时氧自由基产生，促进胃壁黏液分泌等机制有关。
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