目的 探讨弥漫性大B细胞淋巴瘤(DLBCL)中bcl-2蛋白表达情况及其与bcl-2基因易位和扩增的相关性.方法 应用免疫组织化学SP法检测59例DLBCL中bcl-2蛋白表达情况,并应用IgH/bcl-2双色双融合探针荧光原位杂交(FISH)检测bcl-2基因的易位和扩增.结果 59例DLBCL中bcl-2蛋白的表达率为67.8%(40/59),bcl-2基因易位和扩增的发生率分别为1.7%(1/59)和22.0%(13/59),bcl-2基因异常(易位或扩增)的总检出率为23.7%(14/59),bcl-2蛋白表达与基因异常之间无相关性(P=1.000).结论 bcl-2基因可能在DLBCL的发病机制中起重要作用,bcl-2蛋白表达的调控机制有待进一步阐明.%Objective To invesligale the bcl-2 prolein expression and ils correlation wilh bcl-2 gene Iranslocalion and amplification in diffuse large B-cell lymphomai DLBCL) . Methods Immunohislochemical SP melhod was used lo deled the bcl-2 prolein expression in 59 cases of DLBCL. Inlerphase fluorescence in silu hybridization (FISH) assay wilh IgH/bcl-2 dual-color dual-fusion probe was performed on paraffin-embeded lymphoma lissue seclions lo deled the bcl-2 gene Iranslocalion and amplification. Results Among 59 DLBCL cases, the expression rale of bcl-2 prolein was 67. 8% (40/59) . The incidences of bcl-2 gene Iranslocalion and amplification were I. 7% (1/59) and 22. 0% (13/59) , respectively. The incidence of bcl-2 gene abnormalities including Iranslocalion and amplification was 23. 7% (14/59) . There was no relationship between bcl-2 prolein expression and bcl-2 gene abnormalities (P = 1. 000) . Conclusion The bcl-2 gene might play an important role in the palhogenesis of DLBCL. The regulatory mechanism of bcl-2 prolein expression remains to be further elucidated.
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