• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Applied Mechanics, Materials and Manufacturing >Correlations of Bcl-2 and Survivin Gene Protein Expressions in Colorectal Cancer
【24h】

Correlations of Bcl-2 and Survivin Gene Protein Expressions in Colorectal Cancer

机译：直肠癌Bcl-2和Survivin基因蛋白表达的相关性

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: In this study, immunohistochemical streptavidinbiotin-peroxidase (S-P method) was used to detect the correlation of gene proteins related to apoptosis, such as Bcl-2 and Survivin in colorectal carcinoma. Methods: Pathological specimens from 46 colorectal cancer patients and 8 normal tissues around the tumor specimens were selected. Immunohistochemical streptavidin-biotin-peroxidase assay was used to detect the expression of Bcl-2 and Surviving protein. Results: In 25 cases of Bcl-2 positive expression, there were 24 cases of Survivin positive expression, accounting for 96%; while there were 8 cases of Survivin positive expression in 21 cases of Bcl-2 negative expression, accounting for 38.1%, showing the expression of Bcl-2 and the expression of Survivin was positively correlated (Pearson's R = 0.627, P<0.01). In 15 cases of well-differentiated colorectal cancer tissues, there were 6 Cases of Survivin positive expression, and the positive rate was 40%; in 17 cases of moderately differentiated colorectal cancer tissues, there were 17 cases, and the positive rate was 100%; in 14 cases of poorly differentiated colorectal cancer tissues, there were 9 cases, and the positive rate was 64.29%. The statistical analysis showed that the Survivin positive expression in the three colorectal cancer tissues presented a significant difference (P <0.01). Results: The positive expression of Survivin protein was directly correlated to that of Bcl-2, which suggested that both of them acted on different stages of apoptosis to promote jointly the development of colorectal cancer in a synergistic way. Colorectal cancer is one of the common malignant tumors in digestive tract, of which the morbidity and mortality are increasing rapidly in the world. Tumor formation is caused by activation of protooncogene and inactivation of anti-oncogene leading to hyperproliferation and disdifferentiation. In this study, immunohistochemical streptavidinbiotin-peroxidase (S-P method) was used to detect the correlation of gene proteins related to apoptosis, such as Bcl-2 and Survivin in colorectal carcinoma, their molecule mechanism was studied, and their significance in the development of colorectal cancer was also explored.
机译：目的：在该研究中，使用免疫组化链霉抗生物素蛋白 - 过氧化物酶（S-P方法）来检测基因蛋白与细胞凋亡相关的相关性，如Bcl-2和结直肠癌中的Survivin。方法：选择来自46名结肠直肠癌患者的病理标本和肿瘤标本周围的8例正常组织。免疫组织化学链霉抗生物素蛋白 - 生物素 - 过氧化物酶测定用于检测Bcl-2和存活蛋白的表达。结果：在25例BCL-2阳性表达中，存在24例Survivin阳性表达，占96％;虽然21例BCL-2阴性表达中存在8例Survivin阳性表达，但占38.1％，显示Bcl-2的表达和Survivin的表达正相关（Pearson的r = 0.627，P <0.01）。在15例良好分化的结肠直肠癌组织中，存在6例Survivin阳性表达，阳性率为40％;在17例中度分化的结肠直肠癌组织中，有17例，阳性率为100％;在14例患者中差异不良癌症组织，有9例，阳性率为64.29％。统计分析表明，三种结直肠癌组织中的Survivin阳性表达呈现显着差异（P <0.01）。结果：Survivin蛋白的阳性表达与Bcl-2的阳性表达直接相关，这表明它们两者都在凋亡的不同阶段作用，以协同方式共同促进结直肠癌的发展。结肠直肠癌是消化道中的常见恶性肿瘤之一，其中，在世界上发病率和死亡率正在增加。肿瘤形成是由原毒激素的激活和抗癌基因的失活导致过度增殖和无分化引起的。在该研究中，使用免疫组织化学链霉抗生物素蛋白 - 过氧化物酶（SP方法）来检测与细胞凋亡相关的基因蛋白的相关性，例如Bcl-2和结直肠癌中的Survivin，它们的分子机制是研究的，以及它们在结直肠癌发育中的重要性癌症也探讨了。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号