In this study, the effect of apigenin (AP) on acrylontrile-(ACN) induced lipid peroxidation and DNA damage of sperm in male rats were examined and the possible mechanisms were discussed. 50 healthy adult male Sprague-Dawley rats were randomly divided into five groups: control group (corn oil), ACN group (50 mg•kg-1 ACN), low-dose AP group (50 mg•kg-1 ACN+234 mg•kg-1 AP), high-dose AP group (50 mg•kg-1 ACN+468 mg•kg-1 AP) and NAC group (50 mg•kg-1 ACN+300 mg•kg-1 NAC). The rats were intragastrically administered daily with 5 mL•kg-1 (boby weight) for 13 weeks, 6 days per week. The sperm samples were collected for the determina-tion of the content of reactive oxygen species (ROS) and malondialdehyde (MDA), the activity of superoxide dis-mutase (SOD), and the DNA damage. The result showed in comparison with control group, the contents of ROS and MDA, the tail DNA%, tail length, tail moment and Olive tail moment were increased significantly in ACN group, low-dose AP group, high-dose AP group and NAC group, while the activity of SOD was decreased signifi-cantly in these groups (P<0.05). In contrast, the difference among low-dose AP group, high-dose AP group, NAC group and ACN group had no statistical significance (P>0.05). These data indicate that regardless the presence of AP and/or NAC, ACN could induce sperm lipid peroxidation and DNA damage in male rats.%分析芹菜素(apigenin, AP)对丙烯腈(acrylonitrile, ACN)引起的大鼠精子脂质过氧化和DNA损伤的影响,并探讨其可能的机制。将50只SPF级SD成年雄性大鼠随机分为阴性对照组(玉米油)、ACN组(50 mg•kg-1 ACN)、低AP组(50 mg•kg-1 ACN+234 mg•kg-1 AP)、高AP组(50 mg•kg-1 ACN+468 mg•kg-1 AP)、N-乙酰半胱氨酸(N-acetylcysteine, NAC)组(50 mg•kg-1 ACN+300 mg•kg-1 NAC),以5 mL•(kg bw)-1灌胃染毒,1次•d-1,6 d•周-1,连续13周。检测大鼠精子活性氧(ROS)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性以及精子DNA损伤情况。结果发现,ACN组、低AP组、高AP组、NAC组精子ROS、MDA含量显著升高,SOD活力显著降低,精子尾部 DNA含量百分比、尾长、尾距、Olive尾距均显著增高于对照组(均P<0.05)；而低AP组、高AP组、NAC组精子ROS、MDA含量、SOD活性和精子DNA损伤情况与ACN组相比差异均无统计学意义(P>0.05)。提示ACN可引起大鼠精子脂质过氧化和DNA损伤,而AP、NAC对其无干预作用。
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