为了研究P. pastorisMpr1的生理特性，在E. coliJM109胞内中成功表达来源于P. pastorisGS115的Mpr1酶，并利用响应面分析法对诱导温度、IPTG诱导浓度、起始诱导OD进行发酵优化，酶活达到（610.3±9.5）mU/mL。酶学性质显示，Mpr1酶的最适反应pH范围为7.0-7.5，最适反应温度是30℃。通过分析重组表达Mpr1菌株和对照菌株的培养过程，显示重组菌株的生长能力显著增强，原因是Mpr1降低了细胞内的ROS水平。%Due to the nature inP. pastorismethanol metabolism, it suffers much more ROS oxidative stress. There is one Mpr1 enzyme inP. pastoris. It plays significant physiological roles in ROS oxidative stress resistance ability and related research is still blank. For a detailed study about the physiological characteristics ofP. pastoris Mpr1, Mpr1 fromP. pastorisGS115 had been successfully expressed inE.coli JM109. Fermentation optimization of recombinant cell was studied from induction temperature, IPTG induction concentration, Initial induction OD by using Response Surface Analysis, activity reached(610.3±9.5)mU/mL. Enzymatic properties showed that the optimal pH of Mpr1 was about 7.0 to 7.5, the optimum temperature of Mpr1 was 30℃. In order to explore the nature of Mpr1, PQE30-E.coli JM109 strain and PQE30-Mpr1-E. coli JM109 strain were cultured under the same fermentation conditions in this experiment. The results showed that the growth capacity of the recombinant strain was stronger. The reason is that Mpr1 reduces levels of intracellular ROS.
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