随机采集的638份食品样品中沙门氏菌总检出率为9.7%（n=62株），共检出16种不同的血清型，其中最常见的为鸭沙门氏菌。受试菌株对磺胺甲基异噁唑、复方新诺明、链霉素和环丙沙星的耐药率较高。16株耐环丙沙星沙门氏菌按GyrA和ParC喹诺酮耐药决定区（QRDR）不同氨基酸替代组合可分为5种突变型，其中GyrA亚基发生Ser83Phe和Asp87Gly变异，同时ParC亚基发生Ser80Arg变异为最常见的突变类型。62株食源性沙门氏菌中，qnr基因阳性的菌株共7株，占受试菌株的11.3%。qnrA和qnrS基因阳性菌株分别有2株和5株，没有菌株携带qnrB、qnrC和qnrD基因。aac（6'）-Ib基因阳性菌株共有8株，其中3株经确认为携带其变体基因aac（6'）-Ib-cr。结果表明，新乡市食源性沙门氏菌血清型分布呈多样性，耐药状况较为严重，并且一些菌株携带质粒介导喹诺酮耐药（PMQR）基因。%A total of 638 food samples were collected randomly to determine the prevalence of Salmonella. The overall percentage of Salmonella prevalence was 9.7%(n=62). Among 16 different serotypes identified, S. Anatum was most common. The isolates were frequently resistant to sulfamethoxazole, trimethoprim/sulfamethoxazole, streptomycin, and ciprofloxacin. Five different types of amino acid substitutions were identified in quinolone resistance-determining region(QRDR)of GyrA and ParC of 16 ciprofloxacin-resistant isolates. Double mutations of Ser83Phe and Asp87Gly in GyrA accompanied by an additional mutation of Ser80Arg in ParC were found most frequently. The qnr genes were present in seven(11.3%)of 62 isolates, and among them, two isolates carried qnrA and five carried qnrS. qnrB, qnrC, and qnrD were not detected in the isolates in this study. Eight isolates were positive for aac(6')-Ib, of which three isolates carried the-cr variant. The results suggested the diversity of serotype distribution, serious situation of antimicrobial resistance and the presence of plasmid-mediated quinolone resistance(PMQR)genes in foodborne Salmonella from Xinxiang.
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