This paper summarized the amino acid production capacity , production and export volumn of China in 2017 . The breeding technologies of amino acid producing strains were elucidated . The traditional strain breeding technology has not been out-of-date . Instead , it should be integrated into the study of reverse metabolic engineering .Metabolic engineering has achieved great progress through many years'development ,and moved into systematic metabolic engineering .Meanwhile ,the evolutionary metabolic engineering has shown great potential .The construction of industrial strains is often operated at the genome level .Therefore ,this paper introduces the genome editing technologies of Escherichia coli and Corynebacterium glutamicum . Finally ,precise metabolic regulation technologies are briefly introduced ,and a case study is also provided .%综述了2017年我国氨基酸产能、产量及出口情况,系统介绍了氨基酸生产菌株选育技术 .常规育种技术尚不过时,其应与反向代谢工程研究相结合 .代谢工程育种技术经过多年的发展,已经取得了长足的进步,从常规代谢工程发展为系统代谢工程,同时进化代谢工程也展现出巨大的应用价值 .工业化菌株构建需在基因组水平操作,介绍了氨基酸生产菌种大肠杆菌和谷氨酸棒杆菌基因组编辑技术的现状 .最后,简要介绍了精确代谢调控技术,包括基因表达的强化和弱化策略,并给出了应用实例 .
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