Bacterial infection and the question(such as inflammation, fever and pain) caused by bacterial infection on the surface of implant metal material have become a major concern. In this study, by ultilizing electrodeposition, a colloidal particles based antibacterial coating with redox-responsive drug releasing property was prepared aiming at improving the adhesion of bacteria and subsequent formation of biofilms onto implant surfaces. Firstly, redox-responsive amphiphilic random copolymer poly-1-bromododecylmethylethylenedi-ethacrylate quaternary ammonium salt-co-N-[2-(pyridine-2-disulfonyyl)-ethyl]-acrylamide-co-methyl acrylate ( PPDM) was synthesized by a conventional free radical polymerization using bactericidal moiety 1-bromodode-cylmethyldimethylethyl quaternary ammonium salt ( DMAC12 ) , redox-responsive moiety N-[ 2-( pyridine-2-disulfonyyl)-ethyl]-acrylamide ( PDSA ) , and methyl methacrylate ( MMA ) as monomers. Proton nuclear magnetic resonance spectrometer ( 1 H NMR ) , Fourier transform infrared spectroscopy ( FTIR ) and gel permeation chromatography( GPC) were used to confirm the synthesis of PPDM. Then, the resultant copolymer and drug paracetamol( PCTM) were self-assembled together into PCTM-loaded colloidal particles( CPs) . The size, morphology and drug-loading efficiency of CPs were characterized by zeta potential and nanoparticle size analyzer, transmission electron microscope ( TEM ) and UV irradiation. Finally, the PCTM-loaded PPDM coating was prepared on the surface of 316L stainless steel via electrodeposition using PCTM-loaded CPs as building blocks. The chemical composition, morphology and phase of the obtained coating were analyzed using ATR-FTIR, X-ray diffractometer(XRD) and scanning electron microscope(SEM). The results demonstrated that due to the existence of bactericidal cations, PCTM-loaded PPDM coating showed excellent bactericidal function against both Gram negative bacteria E. coli and Gram positive bacteria S. aureus. Moreover, the trigger release of PCTM in coating materials could be realized when glutathione ( GSH) existed. When the concentration of GSH was 10 mmol/L, the amount of released PCTM was about 0. 9 mg which is three times more than that of released without GSH. Considering the antibacterial and redox-responsive drug releasing property, the PCTM-loaded PPDM coating has a great potential application onto the surface of impalnt metal. Furthermore, the entire strategy may be used in other implantations to creat functional coating to improve the bacterial infection.%制备了具有氧化还原响应性的无规共聚物聚{1-溴十二烷基甲基丙烯酸二甲氨基乙酯季铵盐-co-N-[2-(吡啶-2-二硫烷基)-乙基]-丙烯酰胺-co-甲基丙烯酸甲酯}(PPDM),采用核磁共振波谱、 凝胶液相色谱(GPC)及傅里叶变换红外光谱对聚合物的结构进行表征;通过选择性溶剂法制备负载有小分子药物扑热息痛(PCTM)的PPDM载药胶体粒子,通过纳米粒度仪、透射电子显微镜(TEM)及紫外分光光度计等对胶体粒子的粒径、形貌和载药率进行了表征;最后利用电泳沉积技术将上述载药胶体粒子沉积到316L不锈钢表面制备涂层,对涂层的化学组分、表面形貌、抗菌性能、响应释药性能及细胞毒性等进行了表征.研究结果表明,以响应性载药胶体粒子为构筑单元,利用电泳沉积法可以在316L不锈钢表面制备具有响应性释药功能的抗菌涂层,该涂层在通过季铵盐杀死细菌的同时可在还原性环境中响应性释放药物扑热息痛,从而改善由细菌感染所引起的疼痛及发热等症状.
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