Adipic dihydrazide-modified PLGA(PLGA-ADH) and 3-amino-1,2-propanediol-modified PLGA were prepared by the activation of poly-L-glutamic acid(PLGA), the latter was further oxidized by sodium periodate to obtain aldehyde-modified PLGA(PLGA-CHO).Then, the two precursors including PLGA-ADH and PLGA-CHO were mixed to construct the injectable hydrogel by Schiff base reaction.The effects of solid content on the gelation time, swelling behavior, mechanical properties, in vitro degradation properties and microstructure of PLGA were investigated.Encapsulation of rabbit chondrocytes within the hydrogels showed cytocompatibility of the injectable hydrogels.A preliminary study exhibited in vivo injectability and mechanical stability of the hydrogels.The PLGA injectable hydrogel has a good application prospect in the field of tissue engineering.%通过活化改性聚L-谷氨酸(PLGA)制备酰肼化PLGA(PLGA-ADH)和3-氨基-1,2-丙二醇改性的PLGA(PLGA-OH), PLGA-OH经高碘酸钠氧化制得醛基化PLGA(PLGA-CHO), 以PLGA-ADH和PLGA-CHO为前驱体, 通过席夫碱交联反应构建了PLGA可注射水凝胶.研究了酰肼化和醛基化改性前后PLGA的结构变化, 考察了固含量对水凝胶成胶时间、 溶胀行为、 机械性能、 体外降解性能、 药物释放行为及微观形貌等的影响, 并进行了初步的细胞培养实验及裸鼠皮下注射成胶实验.结果表明, 该PLGA可注射水凝胶在组织工程领域具有良好的应用前景.
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