The magnetic beads loaded with high-density Hepatitis B Surface Antigen (HBSAg) detection probes were prepared, and the properties of the beads for HBsAg detection were also studied. The immunomagnetic beads coupled with HBsAb were prepared by chemical bond. Then, immunomagnetic beads were used for detecting HBSAg and were obtained a favorable effect. In order to improve the accuracy and sensitivity of detection, the preparation process of immunomagnetic beads was optimized, including amination and coupling process. Through the optimization, the amino content of magnetic beads was 2.71 mmoL/g, unit magnetic beads bonded 108.36 μg/mg antibodies and the coupling efficiency was 77.40％. The activity of HBsAg on magnetic beads was detected by the method similar to Enzyme-Linked Immunosorbent Assay (ELISA).Compared with common ELISA technology, magnetic beads loaded with high-density HBSAg have higher sensitivity.%通过化学键连接的方法制备出表面偶联乙肝抗体的磁性复合微球(亦称"乙肝抗原检测免疫磁珠"),并用于乙肝检测.结果表明,该方法具有较好的检测效果.通过优化得到氨基磁性复合微球的氨基含量为2.71mmoL/g,单位磁珠抗体偶联量为108.36μg/mg,偶联效率为77.40%的负载高密度乙肝检测探针的磁珠.采用类"双抗原夹心酶联免疫法"对乙肝抗体的活性及优化效果进行了检测.通过性能检测比较,磁珠法检测灵敏度高于普通酶联免疫法.
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