以SiO2凝胶膜和蛋白质交互组装法固定血红蛋白(Hb),对其进行了电化学和电催化研究.首先制备碳纳米管/金纳米粒子复合材料修饰的MWNTs-Au/GC电极,为防止蛋白质在电极表面流失,将Hb和自制的SiO2凝胶膜交替滴涂到电极表面,得到SiO2/Hb层层组装膜修饰电极,即{SiO2/Hb }n/MWNTs-Au/GC电极,n=2为优化层数.Hb在{SiO2/Hb}2/MWNTs-Au/GC电极上仍能保持其特有的生物活性,并能与电极进行稳定快速的电子直接转移,同时表现出过氧化物酶特性,对H2O2具有良好的生物电催化还原能力.%Protein direct electrochemistry study and its electro-catalysis analysis were introduced, involving layer-by-layer alternate assembly of hemoglobin ( Hb)and SiO2 gel film onto electrode surface. The electrode was firstly modified with the nano-compound of multi-walled carbon nanotubes and Au nanoparticles, I. E. , MWNTs-Au/GC electrode. Then Hb and SiO2 gel film were alternately spread onto the electrode surface to stabilize the protein and its amount and bioactivity, thus resulting {SiO2/Hb}n/MWNTs-Au/GC electrode. Under the optimized n =2, the Hb on the { SiO2/Hb}2/MWNTs-Au/GC electrode displayed its intrinsic bioactivity well, transferring electrodes with electrode and catalyzing H2O2 reduction as one perqxidaze enzyme.
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