The determination method of five different industrial dyes ( auramine O, orange G, orange II, basic orange II, metanil yellow) in five different Chinese medicines was establish by HPLC. The experimental conditions were listed as below:the Kromasil C18 column(4. 6 mm×250 mm,5 μm)was used and the detection wavelength was at 430 nm;the column temperature was at 30℃;the mobile phase consisted of methanol-water-20mmol·L-1 ammonium acetate(gradient elution);the flow rate was 1. 0 mL·min-1. The limit of detection(3S/N)for the five dyes were found to be 0. 05、0. 5、0. 2、0. 1和0. 2 μg·mL-1 respectively. The average re-coveries were 92. 28~109. 1%,and RSD were 0. 56~1. 5%. Auramine O was found in Radix Scutellariae and Cattall Pollen. The results indicate that the method can be used for the quality control of the dyes in five kinds of traditonal Chinese medicines.%建立了反相高效液相色谱法同时测定五种中药中的五种工业染料(金胺O、橙黄G、金橙II、碱性橙II和皂黄)的方法。色谱柱为Kromasil C18(4．6 mm×250 mm,5μm)；检测波长为430 nm；温度30℃；流动相为甲醇-水-乙酸铵(20 mmol·L-1),梯度洗脱；流速1．0 mL·min-1。金胺O、橙黄G、金橙II、碱性橙II和皂黄的方法检出限分别为(0．05、0．5、0．2、0．1和0．2μg·mL-1)(S/N=3)。样品的平均加标回收率为92．28~109．1%, RSD为0．56~1．5%。在供试品蒲黄和黄芩中检出工业染料金胺O。方法可用于五种常见中药中相关非法添加染料的检测。
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