建立了硅钼蓝分光光度法测定卡托普利的方法,详细探讨了硅钼蓝光度法测定卡托普利的各种影响因素。结果表明,卡托普利分子中的巯基(-SH)可以定量地将硅钼黄还原为硅钼蓝,通过测定硅钼蓝的吸光度从而间接地测定卡托普利的含量。显色体系最大吸收波长为690 nm,卡托普利质量浓度在4．240~55．12μg ·mL-1范围内与A呈良好的线性关系,线性回归方程为 A=0．0044+10．259ρ( mg·mL-1),线性相关系数 r=0．9998。方法用于测定实际药品中卡托普利的含量,结果与药典法相符。%A novel method for the spectrophotometric determination of captopril by silicomolybdenum blue has been established. The various effect factors on the spectrophotometric determination of captopril by silicomolybdenum blue were discussed in detail. The results showed that silicomolybdenum yellow could be reduced to silicomolybdenum blue by hydrosulfuryl(-SH) in captopril mole-cule,and the content of captopril was determinated indirectly through determinating the absorbance of the silicomolybdenum blue. The maximum absorption wavelength of chromogenic system is 690 nm,good linear relationship is obtained between the absor-bance and the concentration of captopril in the range of 4. 240~55. 12 μg··mL-1 ,and the equation of the linear regression is A=0. 0044+10. 259ρ( mg·mL-1 ) with a linear correlation coefficient is 0. 9998. This proposed method has been applied to determinate of captopril in real pharmaceuticals,and the results agree well with pharmacopoeial method.
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