采用溶胶-凝胶法,以N-叔丁氧羰酰-L色氨酸为模板分子,3-氨丙基三乙氧基硅烷为功能单体,四乙氧基硅烷为交联剂,制备了分子印迹整体柱.对制备过程影响较大的因素温度、酸度等条件进行了优化.用氮气吸附法对聚合物孔径分布进行了表征,聚合物上含有模板分子形成的介孔,平均孔径63左右.将印迹柱用于N-叔丁氧羰酰-DL-色氨酸分析,实现了对映体的快速基线分离,分离度为1.31.%Using N-[ (tert-Butoxy)carbonyl]-L-tryptophan as the template molecule, 3 - aminopropyl triethoxysilane as a functional monomer, tetraethyl orthosilicate as crosslinker, a type of molecular imprinted monolithic column was prepared with sol-gel method. The main factors for preparation ,such as temperature, acidity and other conditions ,were optimized. Pore size distribution of the polymer was characterized by nitrogen adsorption. Polymer contained mesoporous of the template molecule. The average diameter was about 63A. Imprinted monolithic column which was preparated was used as analysis of N-Boc-DL-tryptophan and achieved rapid baseline separation for enantiomers. Separation was 1.31.
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