A new phenanthroline derivative was synthesized and the product was characterized by means of IR,1H-NMR, 13 C-NMR and ESI-MS spectrometries.Stabilization effect of the target compound on the human telomeric G-quadruplex DNA ( AG3 ( T2 AG3 )3) was further studied using FRET melting assay and UV-visible spectra in 10 mM Tris-HCl buffer ( pH 7.4) containing 100 mM NaCl.The result showed an increase in the melting temperature ( ΔTm ) of 7.9 ℃ for G-quadruplex in the presence of 2.0 μM compound, suggesting that this compound can recognize and stabilize G-quadruplex structure, and their association constant is(2.0 ±0.06) ×106 M-1.%设计合成了一种新型邻菲罗啉衍生物,用IR,<'1>H-NMR,<'13>C-NMR和质谱进行了结构表征.在包含100mM NaCl的Tris-HCl(pH 7.4)缓冲溶液中,利用荧光共振能量转移熔点(FRET-melting)分析以及紫外可见光谱研究了此化合物对人端粒G-四链体DNA(AG<,3>T<,2>AG<,3>),)的稳定作用.发现2.0μM的化合物可以使G-四链体的熔点温度升高(△T<,m>)7.9℃,表明新合成的化合物具有识别并稳定人端粒G-四链体DNA的作用,二者的结合常数为(2.0±0.06)×10<'6>M<'-1>.
展开▼
