We separated a strain of marine fungus Penicillium oxalicum T4-6 from the sediments of Bohai Sea.Through preliminary optimization of fermentation conditions,we obtained eleven compounds by silica gel column chromatography,Sephadex LH-20 gel column chromatography,reversed phased silica gel column chro-matography,preparative thin layer chromatography,and preparative high performance liquid chromatography. Moreover,we identified their structures by 1HNMR and 13CNMR as secalonic acid D(Ⅰ),ω-hydroxyemodin (Ⅱ),isorhodoptilometrin(Ⅲ),oxalicine B(Ⅳ),decaturin B(Ⅴ),decaturin F(Ⅵ),penipanoid C(Ⅶ),cyathis-terone(Ⅷ),herbarulide(Ⅸ),dankasterones A(Ⅹ),and volemolide(Ⅺ).Among them,compound Ⅵ shows ob-vious anti-microalgae activities against Heterosigma akashiwo and Chattonella marina.Additionally,compoundsⅠ and Ⅱshow good anti-microalgae activities against Chattonella marina,and compoundⅠpossesses antibacte-rial activities against Bacillus subtilis and Bacillus anthracis.%从采集自渤海沉积物样品中分离得到一株海洋真菌草酸青霉 T4-6,对其前期发酵条件进行调控和扩大发酵,所得发酵产物利用硅胶柱层析、Sephadex LH-20凝胶柱层析、反相硅胶柱层析、制备薄层层析、制备高效液相色谱等分离手段得到11个单体化合物,通过1HNMR、13CNMR 等鉴定其分别为 secalonic acid D(Ⅰ)、ω-hydroxyemodin(Ⅱ)、isorhodoptilometrin(Ⅲ)、oxalicine B(Ⅳ)、decaturin B(Ⅴ)、decaturin F(Ⅵ)、penipanoid C(Ⅶ)、cyathisterone(Ⅷ)、herba-rulide(Ⅸ)、dankasterones A (Ⅹ)、volemolide (Ⅺ).其中,化合物Ⅵ对赤潮异弯藻和海洋卡盾藻的生长具有较强的抑制作用,化合物Ⅰ、Ⅱ对海洋卡盾藻的生长具有较好的抑制作用,化合物Ⅰ对枯草杆菌和炭疽杆菌具有良好的抑制作用.
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