猪血凝性脑脊髓炎是由猪血凝性脑脊髓炎病毒(PHEV)感染引起的一种以仔猪呕吐、衰竭或显著神经症状为主要特征的急性、高度接触性传染病,给世界养猪业造成了严重经济损失.本文阐述了PHEV的发现、分类地位、形态特征、理化特性、基因组结构及功能、培养特性、流行病学与致病性等,介绍了PHEV检测方法,包括病毒分离与鉴定、血凝/血凝抑制试验等6种血清学方法,以及RT-PCR等3种分子生物学方法,以期为该病诊断和防控提供参考.%Hemagglutinating encephalomyelitis(PHE)is an acute and highly contagious disease caused by Porcine hemagglutinating encephalomyelitis virus(PHEV),which is characterized by piglet vomiting,exhaustion or significant neurological symptoms. It has caused serious economic loss to the world swine industry. In this paper, the discovery,classification,morphological features,physicochemical characteristics,the genome structure and function,culture features,epidemiology and pathogenicity of PHEV were introduced,and detection methods of PHEV were analyzed,including virus isolation and identification and 6 kinds of serological methods,such as hemagglutination/hemagglutination inhibition test,and 3 kinds of molecular biological methods(including RT-PCR) were also introduced,so as to provide reference for better diagnosis and prevention of this disease.
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