To investigate the information of drug resistance phenotypes and disinfectants resistance of pig source E. Coli O157: H7 isolated from Guangxi,the drug sensitive and disinfectants resistance were assayed. Meanwhile, 3 aminoglycoside antibiotic resistance genes,αPh(3)-Iia ,aadA and ααdB were amplified by PCR. The results of drug sensitive to 27 antibiotics showed that 5 strains were sensitive to Florfenicol,Ceftriaxone,Cefoxitin and Cefotaxime. The rate of drug resistance to Rox-ithromycin,Polymyxin B,Rifampin,Lincomycin, Amoxycilin, Ampicilin and Cephalothin were 100%. The rate of drug resistance to other antibiotics,such as Trobicin,Streptomycin,Cefradine were between 20% and 60%. Among of 5 strains, 1 strain, 1 strain and 3 strains were resistances to 23,11 and 9 antibiotics,respectively. The results of PCR confirmed that genome of 5 strains contained aminoglycoside antibiotic resistance genes. Antibiotic resistance genes had a certain correlation with drug resistance phenotypes. The results of disinfectants resistance showed that 5 strains were resistance to Povidone Iodine solution, Bromogeramine solution,Glutaraldehyde solution,double quaternary ammonium salt-iodine disinfection liquid and compound of peracetic acid,except dischloroisocyanuric acid sodium chloride powder. Our results will provide information to prevention and control of E. Coli O157 : H7 in Guangxi.%为了调查广西猪源大肠杆菌O157∶H7分离株的耐药表型和消毒剂抗性情况,本研究测定5株猪源大肠杆菌O157∶H7广西分离株的药物敏感性和消毒剂抗性,并应用PCR对5株细菌的耐氨基糖苷类抗生素基因:氨基糖苷磷酸转移酶基因aph(3) -Iia、乙酰转移酶基因aadA和aadB进行扩增.27种抗菌药物的敏感结果表明,5株菌株只对氟苯尼考、头孢曲松、头孢西丁和头孢噻肟敏感;对罗红霉素、多黏菌素B、利福平、林可霉素、阿莫西林、氨苄西林和头孢噻吩的耐药率为100％;对壮观霉素、链霉素、头孢拉定等药物的耐药率在20％～60％之间.在5株大肠杆菌O157∶H7广西分离株中,分别有1株、1株和3株菌株对23种、11种和9种抗菌药产生耐药性.PCR结果证实,5株细菌携带有耐氨基糖苷类抗生素基因,耐药基因的存在与其耐药表型有一定的相关性.消毒剂抗性结果显示,5株细菌对聚维酮碘溶液、新洁尔灭消毒液、稀戊二醛溶液、双季铵盐-碘消毒液、复方过氧乙酸具有抗性,只对二氯异氰尿酸钠粉敏感.研究结果对预防和控制广西大肠杆菌O157∶H7提供了数据.
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