对唾液乳杆菌Ls04对小鼠肠道微生物区系和肠黏膜分泌型免疫球蛋白A(SIgA)质量浓度的影响及其持续作用效果进行了研究.将100只SPF雄性昆明白小鼠随机分为Ls高、中、低剂量组及对照组,进行15 d灌胃,将灌胃前、灌胃15 d各处理组小鼠,及灌胃结束后第3,7,14天Ls中剂量组小鼠断颈处死,刮取其大肠黏膜、小肠黏膜,分别进行肠道中乳杆菌、双歧杆菌、肠杆菌、肠球菌和产气荚膜梭菌的计数及肠黏膜SIgA质量浓度的测定.结果表明,灌胃Ls04能显著提高小鼠肠道中乳杆菌、双歧杆菌数量,降低肠杆菌、肠球菌、产气荚膜梭菌数量,且Ls中剂量为最适;灌胃结束后14d,小鼠肠道中乳酸菌、双歧杆菌数量仍高于灌胃前数量,且肠杆菌仍被显著抑制;灌胃15 d,Ls中剂量组小鼠肠黏膜SIgA质量浓度显著升高,且显著高于Ls高剂量组;灌胃结束后小鼠肠黏膜SIgA质量浓度至14 d显著降低,但仍显著高于灌胃前水平.结果表明,唾液乳杆菌Ls04停用后仍可显著增殖肠道有益菌并抑制致病菌,同时能够有效提高肠黏膜SIgA含量,刺激机体免疫应答.%The objective of this study was to determine the effects of Lactobacillus salivarious Ls04 on the intestinal microflora and the mucosal SIgA content in mice.Totally 100 SPF male mice were randomly assigned to 4 groups:Ls high-,Ls medium-and Ls low-dose and control groups,and the mice were orally administrated with different doses of Ls04 and water for continuous 15 d,respectively.The mice in all treatments before and at 15 d of oral administration,and the mice in the Ls medium-dose group at 3 d,7 d and 14 d after the termination of oral administration were sacrificed.The populations of Bifidobacterium,Lactobadllus,Enterococcus,Enterobacteriaceae,and Clostridium perfringens and the SIgA contents were determined in the large intestine tissue and in the small intestine mucosa,respectively.Ls04 significantly increased the numbers of Lactobacillus and Bifidobacteria,and significantly reduced the counts ofEnterobacteriaceae,Enterococcus and C.perfringens,and compared with Ls high-dose group,Ls medium-dose group exhibited no significant differences in the numbers of Bifidobacterium,Enterobacteriaceae,and Enterococcus in this experiment.After the end of the oral administration in the Ls medium-dose treatment,the numbers of Bifidobacteria and Lactobacillus were decreased significantly (P＜0.05),but still the numbers at 14 d were higher than those before oral administration.Enterococcus were still significantly inhibited at 14 d.During oral administration,the content of the mucosal SIgA were significantly increased in the Ls medium-dose group,and although the SIgA content was significantly decreased after the end of the oral administration,the SIgA content at 14 d was still higher than that before the oral administration.Our findings indicated that Ls04 could effectively increase the numbers of the beneficial bacterial and inhibit the enteropathogenic bacteria,and effectively improve the secretion of the mucosal SIgA,stimulating the immune response in mice.
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