The mannose phosphotransferase system IID mutant of L. plantarum WQ0815 has been constructed by homologous recombination technology, which is sensitive to plantaricin LB-B1. The sensitivity of mutants to plantaricin LB-Bl was determined by the well-diffusion assay, which showed that interruption of IID gene led to resistance of L. plantarum WQ0815 against class Ila bacteriocin. These results showed that the IID component of sensitive strain is direcdy involved in the recognition of the target cell by class Ila plataricin LB-B1.%采用同源重组技术,对Ⅱa类植物乳杆菌素LB-B1敏感的植物乳杆菌WQ0815中甘露糖磷酸转移酶系统(Ell.Man)中的 ⅡD组分进行突变,利用牛津杯法分析了植物乳杆菌WQ0815 ⅡD组分突变前后对植物乳杆菌素LB-B1的敏感性变化.结果表明,突变株对植物乳杆菌素LB-B1产生抗性,说明敏感菌株中Ell.Man的 ⅡD组分是植物乳杆菌素LB-B1发挥抑茵作用时的结合位点.
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