Viable counts of Bifidobacterium is one of the key criterions to improve human body health. The study adopted MRS as basic medium, and four optimal ingredients were chosen and added: Konjac Gum Hydrolyzates, Extractive Juice of Semen Coins, Extractive Juice of Yam, Inulin. Through single -factor test,3 better optimal ingredients were sifted out. To take the viable counts as index, orthogonal experiment was used to select a formula which is the best to enriching high activity bifidobacterium. The experimental result showed that in single— factor test, Konjac Gum Hydrolyzate had the best effect to enriching bifidobacterium among the above 4 factors,and the additive concentration was MRS added with Konjac Gum Hydrolyzates (35 mL/L),with the viable counts being 1.21×109 CFU/mL.Orthogonal test showed that the optimum medium was MRS added with Konjac Gum Hydrolyzates(30 mL/L).Extractive Juice of Semen Coins(25 mL/L), Inulin(20 g/L),in-cubated under anaerobic conditions for 36h at 37℃,with the viable counts being 7.4×109 CFU/mL.%以MRS为基础培养基,选取添加4种不同的增菌因子,即:魔芋胶水解物、薏苡仁浸提液、山药浸提液、菊粉,以单因素实验筛选出较优的3种增菌因子,通过正交实验以活菌数为活力评价指标,确定最佳增菌配方.结果表明,单因素实验中,魔芋胶水解物增菌效果最佳,当其在MRS培养基中添加体积分数为35 mL/L时,双歧杆菌的活菌数可达1.21×109 mL-1;正交实验所得最佳增菌配方为:MRS+魔芋胶水解物(30 mL/L)+薏苡仁浸提液(25 mL/L)+菊粉(20g/L),37℃厌氧培养36 h,活菌数可达7.4×109 mL-1.
展开▼
