A nested-PCR assay for detecting brucella infection in milk was developed by means of designing outward-directed and inward-directed primers according to brucella's genome sequences, and optimizing the DNA extraction method, .it is suggested that the nested-PCR assay for Brucella with high specificity and sensitivity are very suitable for detecting brucella infection of milk in laboratory.%研究根据GenBank公布的牛布鲁氏菌基因序列设计内、外向引物,优化牛奶样品中布鲁氏菌基因组DNA提取方法,建立了牛奶布鲁氏菌套式PCR方法.试验建立的布鲁氏菌套式PCR检测方法,具有高度的特异性和敏感性,适用于布鲁氏菌的实验室快速检测.
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