以牟氏角毛藻为研究对象,采用半连续培养法,研究了不同氮浓度条件下牟氏角毛藻对2,2’,4,4’,5-五溴联苯醚富集.结果表明,牟氏角毛藻对多溴联苯醚的富集与氮营养条件有关,随着氮浓度的增加,富集量和富集因子均呈降低趋势.氮浓度为0,128,512μmol/L条件下,单位藻细胞的富集量分别为2.84,2.38,1.91ng/106 cells,单位体积藻液富集量分别为0.19,0.16,0.12ng/mL.富集因子的变化趋势相似,氮为0μmol/L时单位总脂富集因子(BCFlipid)为238.19×104(ng/g)/(ng/mL),随着氮浓度的升高,BCFlipid明显降低.相关性分析表明,单位体积藻液富集量、单位藻细胞富集量和BCFlipid等均与氮浓度呈现显著的负相关关系(r＞-0.8,P＜0.05),而富集量和富集因子与单位藻细胞和单位体积藻液总脂含量存在显著的正相关关系(r＞0.8,P＜0.05).%The bioconcentration of 2,2',4,4',5-pentabromodiphenyl ether (BDE-99) by Chaetoceros muelleri in semi-continuous culture under different nitrogen concentrations was studied. The bioconcentration of BDE-99 by Chaetoceros muelleri was related to the nitrogen concentrations. With the increase of nitrogen concentrations, the bioconcentration content and bioconcentration factor declined. When the concentration of nitrogen was 0, 128, 512 umol/L, the bioconcentration content per cell was 2.84, 2.38, 1.91 ng/106 cells, respectively; and the bioconcentration content per algal culture was 0.19, 0.16, 0.12 ng/mL, respectively. When nitrogen was 0 umol/L, BCFlipid was 238.19><104 (ng/g)/(ng/mL). Pearson correlation analysis indicated the bioconcentration content per cell, algal culture and BCFupid exhibited significantly negative correlation with nitrogen concentrations (r>-0.8, P<0.05). On the other hand, the bioconcentration content and BCFs presented significantly positive correlation with total lipids (r>0.8, P展开▼
