Objective:To determine the effects of paeonol on the apoptosis and proliferation in human mel-anoma cell line A375 and M14. Methods: The anti-proliferative activity of paeonol in A375 and M14 cells was investigated by CCK8 assay. After A375 and M14 cells were treated by 1.25 mmol/L, 2.5 mmol/L or 5 mmol/L paeonol for 24 h, flow cytometry was used to detect the cell cycle and Annexin V-FITC and propidi-um iodide ( PI) double staining was taken to detect the apoptosis rate of A375 and M14 cells, respectively. The morphological changes of A375 and M14 cells treated by 5 mmol/L paeonol for 24 h were observed by Ho-echest 33258 staining. Results:Compared with untreated A375 and M14 cells, an increase of cell population in G2/M phase was observed in paeonol-treated cells. After treatment of 0 mmol/L, 1. 25 mmol/L, 2. 5 mmol/L and 5 mmol/L paeonol for 24 h, the early apoptosis rate of A375 cells was 3.11%±0.53%, 13.74%±1.73%, 25. 95%± 0. 57% and 46. 44%± 0. 81%, respectively, while the rate of M14 cells was 1. 00%± 0.08%, 2.00%±0.01%, 2.99%±0.29% and 14.73%±0.94%, in a dose-dependent manner. After treated with paeonol of 5 mmol/L, there were morphological changes of apoptosis in A375 and M14 cells. Conclusion:Paeonol can inhibite proliferation and induce apoptosis of human melanoma cell line A375 and M14.%目的:确定丹皮酚对体外培养人黑素瘤A375和M14细胞增殖与凋亡的影响。方法:利用CCK8检测黑素瘤A375和M14细胞的增殖抑制率;以流式细胞仪检测细胞周期变化、Annexin-V/PI法观察细胞凋亡的变化;用Hoechst33258染色法观察细胞凋亡的形态变化。结果:细胞周期显示丹皮酚处理过的A375和M14细胞G2/M期比例均高于对照组。以0 mmol/L、1.25 mmol/L、2.5 mmol/L、5 mmol/L丹皮酚作用24 h后,A375细胞的早期凋亡率分别为3.11%±0.53%,13.74%±1.73%,25.95%±0.57%和46.44%±0.81%;M14细胞的早期凋亡率分别为1.00%±0.08%,2.00%±0.01%,2.99%±0.29%和14.73%±0.94%,呈剂量依赖性诱导 A375和 M14细胞凋亡。经5 mmol/L 丹皮酚作用后 Ho-echst33258染色示凋亡细胞形态改变。结论:丹皮酚对黑素瘤A375和M14细胞均具有抑制增殖和诱导凋亡的作用。
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