Objective:To evaluate the platelet (PLT) count performance of Sysmex XN 3000 automatic hematology analyzer.Methods: PLT was counted by Sysmex XN 3000 automatic hematology analyzer with electric impedance (PLT-I), optical (PLT-O) and fluorescence (PLT-F) method to test the precision, linear range, carryover and the red blood cell fragment interference. The count results of PLT-I, PLT-O and PLT-F were compared with those of microscopic count method (PLT-M) respectively.Results: The within batch precision of PLT-F was 1.36%and the between batch precision was 1.93%. The ranges of carryover ratio are from 0 to 0.97%with PLT-F, and the average carry-over ratio was 0.48%. The correlation coefficient (r) was 0.999 in lower, medium and high value specimens. The result showed that the PLT-F method had great ability of anti-interference in RBC fragment interference experiment. There was significant correlation in the results between PLT-F and PLT-M, and the accuracy of PLT-F was higher especially in low PLT specimens group (rPLT-F>rPLT-O>rPLT-I).Conclusion: The measuring item is of high accuracy, good repeatability, quickness and anti- interference for platelet count. When specimens are hemolysis or the PLT count abnormalities, PLT-F or microscopic method are recommend to review the result.%目的:对XN3000血细胞分析流水线新型荧光血小板(PLT-F)计数法的基本性能进行评价.方法:对新型PLT-F计数的总重复性、精密度、携带污染率、线性稀释、抗红细胞碎片干扰以及与显微镜血小板(PLT-M)计数法的相关性等基本性能进行评估.同时进行鞘流电阻抗血小板(PLT-I)计数和光学血小板(PLT-O)检测.结果:总重复性为1.40%,平均批内精密度为1.36%,批间精密度为1.93%,携带污染率为0.00%~0.97%,均值为0.48%;在线性稀释试验中,测试值与理论值的相关系数(r)为0.999;PLT-F对红细胞碎片有较强的抗干扰能力;PLT-F血小板计数值与PLT-M结果相关性良好,且低值血小板组(PLT<100×109/L),3种检测方法的相关系数r值PLT-F>PLT-O>PLT-I.结论:PLT-F法精密度、准确度及线性较好,红细胞碎片的抗干扰能力强.低值血小板计数与PLT-M相关性好,优于PLT-I法.日常检验中使用PLT-I法计数血小板,若计数血小板异常减少或出现散点图异常干扰时,必须用显微镜计数法或PLT-F法进行复查.
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