Objective To establish the quality standard of Gouju Capsules.Methods The main ingredients of Ramudus Uncaria Cum Uncis,Epimedium were identified by TLC.Content of icariin was determined by UV-HPLC.The content of the berberine was determined by HPLC;the column of Agilent ZORBAX SB-C18 (150 mm×4.6 mm,5 μm) was used.The mobile phase was ocetonitrile-water (25∶75),the column temperature of 30℃,at the flow of 1.0 mL/min and detec tion wavelength was 270 nm.Results The spots on TLC plates were distinct,without interference.A good linearity of icariin was in the ranges of 15-150 μg/mL (r=0.9996).The average recovery was 100.9％ (RSD =2.77％).Conclusion The results of this experiment are accurate,simple and easy to control.It can be used as a quality standard for Gouju Capsules.%目的 建立钩菊胶囊的质量标准.方法 薄层色谱法对钩藤、淫羊藿进行定性鉴别;UV-HPLC对淫羊藿苷进行含量测定.采用Agilent ZORBAX SB-C18色谱柱(150 mm×4.6 mm,5μm);流动相:乙腈-水(25∶75);流速:1.0 mL/min;柱温:30℃;检测波长为270 nm.结果 薄层色谱中斑点清晰,阴性均无干扰;淫羊藿苷在15～150 ~mL范围内线性关系良好(r=0.9996),平均回收率为100.9％(RSD=2.77％).结论 本试验结果准确、简便,易控,可作为钩菊胶囊的质量标准.
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