目的：观察热预处理(HA)对小鼠创伤性脑损伤(TBI)的神经保护作用。方法60只小鼠分为正常对照(NT)组、HA组、TBI组、HA+TBI组，每组15只，采用改良自由落体损伤装置建立小鼠TBI模型。观察和比较各组小鼠的麻醉苏醒时间、苏醒后的一般行为学表现、脑组织含水量，脑切片苏木精-伊红(HE)染色观察病理学改变，FJB染色观察神经元变性情况。结果与TBI组比较，HA+TBI组的麻醉苏醒时间缩短，脑组织含水量降低，脑组织染色显示病理损伤程度减轻，变性神经元减少，差异有统计学意义(P<0.05)。HA+TBI组的麻醉苏醒时间长于NT组与HA组，脑组织含水量高于NT组与HA组，差异有统计学意义(P<0.05)。 NT组与HA组的麻醉苏醒时间和脑组织含水量比较，差异无统计学意义(P>0.05)。结论闭合性脑损伤模型构建成功，HA对闭合性脑外伤具有神经保护作用。%Objective To investigate the neuroprotective effects of heat preconditioning (HA) on mice with traumatic brain injury (TBI). Methods 60 mice were divided into normal control (NT) group, HA group, TBI group and HA+TBI group, each group of 15 mice. Free fall injury improved device was adopted to establish the mice model of TBI. The anesthesia recovery time, general behavior, brain tissue water content of mice in each group were compared and ob-served. Pathological change was observed by using HE staining, neuronal degeneration was observed by using FJB staining. Results Compared with TBI group, the anesthesia recovery time of HA+TBI group was shorter, brain tissue water content of HA+TBI group was lower, pathological damage of brain tissue in HA+TBI group was reduced, neuro degeneration of HA+TBI group was decreased, the difference was statistical significance (P<0.05). The anesthesia re-covery time of HA+TBI group was longer than that of NT group and HA group respectively, brain tissue water content of HA+TBI group was higher than that of NT group and HA group respectively, the difference was statistical signifi-cance (P< 0.05). The anesthesia recovery time and brain tissue water content of NT group and HA group were com-pared, with no statistical difference (P> 0.05). Conclusion Closed TBI mice model has established successfully, and HA can offer neuroprotective effects on closed TBI.
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