Objective To establish the method of determination for total flavonoids in Shenzhujing Tablet. Methods The ultrasonic extraction was used, and rutin was used as reference substance, colorimetry was used to determine the total flavonoids in Shenzhujing Tablet from different batches, by flavonoids and sodium nitrite-aluminum nitrate-sodi-um hydroxidc producing color under alkaline conditions. Results Rutin showed good linear relationship in the range of 0.0102-0.0714 mg/mL(r=0.9997), the average recovery was 98.80%, RSD was 0.86%(n=9). Conclusion The method is simple, reproducible, the results are accurately and reliable and can be used to quality control of Shenzhujing Tablets.%目的：建立参竹精片中总黄酮的含量测定方法。方法采用超声法提取，利用黄酮类与NaNO2-Al(NO3)3-NaOH发生显色反应，以芦丁为对照品，采用分光光度法对不同批号参竹精片中总黄酮的含量进行测定。结果芦丁浓度在0.0102~0.0714 mg/mL范围内与吸收度呈良好的线性关系(r=0.9997)，平均回收率为98.80%，RSD为0.86%(n=9)。结论该方法简便，重复性好，结果准确可靠，可作为参竹精片质量控制的方法。
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