Objective To evaluate the methodology of transmitting immune turbidimetry in the determination of im-munoglobulin IgG, IgA and IgM and to evaluate the correlation between the transmitting immune turbidimetry and the scattering turbidimetry. Methods The correlation and deviation analysis was carried out between the transmitting immune turbidimetry and the scattering turbidimetry. Results IgG, IgA and IgM tests showed that the transmitting immune turbidimetry and the scattering turbidimetry methods had good correlation. The deviation between IgG and IgM tests could be accepted on three medical decision levels. The deviation of IgA test was accepted in intermediate and high concentrations, but the deviation between the two methods was large in low concentrations. Conclusion Transmitting immune turbidimetry has reliable methodology in the determination of IgG, IgM and IgA and has certain deviation from the scattering turbidimetry in low concentrations, which should be noticed in clinical application.%目的 对透射免疫比浊法检测免疫球蛋白IgG、IgA、IgM进行方法学评价分析,并对透射免疫比浊法与散射比浊法两种检测方法的相关性进行评估.方法 对透射比浊法与散射比浊法进行相关性及偏差分析.结果 透射比浊法与散射比浊法检测IgG、IgA、IgM的相关性良好.两种方法检测IgG、IgM偏差在三个医学决定水平均可以接受.IgA检测在中间浓度和高浓度时偏差可以接受,而低浓度时两种检测方法的偏差较大.结论 透射免疫比浊法检测免疫球蛋白IgG、IgM、IgA的方法学可靠,与散射比浊法的相关性良好,结果具有可比性.对于IgA的检测,透射免疫比浊法检测低浓度水平与散射比浊法有一定的偏差,临床应用时要予以注意.
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