目的 研究利拉鲁肽对高脂饮食喂养SD大鼠脂肪代谢的影响.方法 将48只雄性SD大鼠随机分为四组,分别予以普通饮食(ND)、高脂饮食(HFD)、高脂饮食加小剂量利拉鲁肽腹腔注射(HFD+SGLP-1)、高脂饮食加大剂量利拉鲁肽腹腔注射(HFD+LGLP-1).喂养8周后检测大鼠血脂、血糖、体重,并检测大鼠肝脏ApoE蛋白表达水平的变化.结果 与ND组比较,HFD组大鼠体重、血脂及血糖水平显著升高,肝脏ApoE蛋白水平显著降低(P ＜ 0.05).而与HFD组比较,HFD+SGLP-1组和HFD+LGLP-1组大鼠体重、血脂及血糖水平明显降低,ApoE蛋白水平明显增高(P ＜ 0.05),但以HFD+LGLP-1组效果最为显著.结论 利拉鲁肽能够显著抑制高脂饮食喂养导致的体重增加,降低血糖及血脂水平,并调节血脂谱.利拉鲁肽改变血脂谱的作用可能与其增加肝脏ApoE蛋白的表达有关.%Objective To investigate the anti-hyperlipidemia effect of Liraglutide on high-fat diet SD rats and its mechanism. Methods Fourty eight male SD rats were randomly separated into four groups accordding to body weight. They were respectively fed with normal diet (ND), high-fat diet (HFD), high-fat diet with small doses of Liraglutide injected intraperi-toneally (HFD+SGLP-1), high-fat diet with large doses of Liraglutide injected intraperitoneally (HFD+LGLP-1). After 8 weeks, the body weight, blood sugar level and lipid profile were measured, the change of ApoE protein expression level in liver were detected by western blot. Results Compared with those in ND group, the body weight, blood-fat level and blood sugar level of HFD group increased significantly, and the ApoE level decreased significantly (P < 0.05). Compared with HFD group, there were significantly lower for the body weight, blood-fat level and blood sugar level and significant high for the ApoE level of HFD+SGLP-1 group and HFD+LGLP-1 group (P < 0.05). But the effect of HFD+LGLP-1 group was most pronounced. Conclusion Liraglutide can obviously restrain high-fat diet induced over-weight, reduce blood-fat and blood sugar levels and regulate lipid profile. The regulatory function of change lipid profile may have a relationship with the improvment of ApoE protein expression in liver.
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