目的 建立益肺通络颗粒有效成分岩白菜素的含量测定方法.方法 采用RP-HPLC法,Agillent高效液相色谱仪,Kromasil 0DS(250 min×4.6 mm,5μm)色谱柱;甲醇-水(20:80)为流动相;流速为1 mL/min;柱温为30℃;检测波长为275 nm;灵敏度为0.05 AUFS.进样量10μL.结果 在该方法中,岩白菜素进样量在0.054～0.540 μg范围内有良好的线性关系,r=0.999 06,平均回收率为98.53%,RSD为1.32%.结论 本方法准确简便,准确,重复性好,可用于益肺通络颗粒中有效成分岩白菜素的含量测定.%Objective To establish a method for determination of bergenin in compound Yifei Tongluo Granules. MethodsrnThe RP-HPLC method was adopted. The Kromasil ODS column (4.6 mm x250 mm, 5 μm was used at the columnrntemperature of 30℃. The mobile phase consisted of methanol-water (20-80) with the flow rate of 1.0 mL/min and therndetection wavelength was 275 nm. Results There was a good linear relationship between the peak area and the sample sizernof bergenin within the range of 0.054 0.540 μg, r = 0.999 06, and the average recovery rate was 98.53% with the RSD ofrn1.32% (n= 6). Conclusion The method is simple, accurate and repeatable, which can be used for the quality control ofrnYifei Tongluo Granules.
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