A RP-HPLC analytical method was developed for determining bergenin in rat urine, feces and tissues. The separation was performed on a C18 analytical column with methanol-water as the mobile phase at a flow rate of 0.8 mL/min. The column temperature was 40℃ and the eluate was monitored at 220 nm. The calibration curve for urine analysis showed a good linearity (r=0.9989) over the range of 0.5-100 μg/mL, and the absolute recoveries were in the range of 90.0%-92.6%. A good linearity (r≥0.9996) for all tested tissues was obtained over the range of 0.3-150 μg/g except for lung, which had a linearity range of 0.5-150 μg/g. The absolute recoveries from tissues were higher than 63.9%. The method was successfully applied to determine the bergenin levels in several biological samples after rats were given bergenin formulation through tail vein injection. The results demonstrated that the amount of bergenin recovered from urine was about 35.36% of the total dose, and that fi'om feces was less than 10%. Bergenin can be quickly and widely distributed in the rat, accumulating high levels in the kidney in comparison to all other tissues investigated. It was not distributed into the brain at any significant level owing to its hydrophilic property.%本文建立了应用反相高效液相色谱测定大鼠尿、粪便和组织中水溶性药物岩白菜素含量的方法.色谱条件为Diamonsil<'TM> C<,18>色谱柱,甲醇-水为流动相,流速0.8 mL/min,柱温40℃,检测波长220 nm.尿中药物浓度在0.5-100μg/mL范围内与峰面积旱良好线性关系(r=0.9989),绝对回收率介于90.0%-92.6%.肺中药物浓度在0.5-150μg/g范围内,其它被测组织中药物浓度在0.3～150 μg/g范围内与峰面积具有良好线性关系(r≥0.9996),各组织中药物绝对回收率均大于63.9%.该方法用于测定大鼠静脉注射岩白菜素后尿液、粪便和组织中药物的浓度,结果表明约有35.36%的服药剂量从其尿液中回收,不足10%的剂量从粪便中回收.原形药物能快速分布到被测组织中,肾中药物分布最多,而脑组织中分布最少,这与药物本身具有较好的水溶性相关.
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