Objective: To establish an HPLC method for content determination of cinobufagin and resibufogenin in Jiuxin Pills. Methods: The column was C18(250 mm×4.6 mm, 5 μm).The mobile phase was acetonitrile-0.5％KH2PO4 (pH=3.2) (50:50)and the flow rate was 1.0 ml/min. The detection wavelength was at 296 nm. Results: Cinobufagin had a good linearity between 0.498-2.490 Vg and resibufogenin had a good linearity between 0.570-2.850μg Conclusion: The method is simple and accurate with good repeatability and can be used for the quality control of cinohufagin and resibufogenin in Jiuxin Pills.%目的:建立救心丸中华蟾酥毒基、脂蟾毒配基的含量测定方法.方法:色谱柱:C18(250 mmx4.6mm,5μm);柱温:40℃;流动相:乙腈-0.5%磷酸二氢钾(pH=3.2)(50:50);流速:1.0 ml/min;检测波长:296 nm.结果:华蟾酥毒基在0.498～2.490μg范围内线性关系良好;脂蟾毒配基在0.570～2.850μg范围内线性关系良好.结论:本方法简便、准确,重复性好,可作为救心丸的质量控制方法.
展开▼
