目的:研究咪达唑仑和丙泊酚对小鼠学习记忆能力的影响.方法:将88只小鼠随机分为8组,每组各11只,分别为:咪达唑仑(腹腔注射15、30、60mg/kg)组,丙泊酚(腹腔注射60、120、240mg/kg)组,生理盐水(腹腔注射10ml/kg)组,和一组不作任何处理的空白对照组.然后用自建的水迷宫测试其对小鼠学习记忆能力的影响.结果:小鼠第一次注射,恢复正常后马上进行水迷宫实验,与注射前相比,咪达唑仑三组潜伏期均延长(其中15 mg/kg组t=2.693,P=0.023;30 mg/kg组t=4.633,P=0.001;60 mg/kg组t=2.550,P=0.029),其他各组潜伏期无显著性差异(P＞0.05).继续注射两天后停药24 h,再进行一次水迷宫实验,与第一次注射前相比,丙泊酚120 mg/kg和240 mg/kg组潜伏期缩短(分别为t=2.447,P=0.034;t=4.619,P=0.001),其他各组潜伏期无明显差异(P＞0.05).结论:咪达唑仑对小鼠的学习记忆能力影响具有瞬时性,不具长期性;丙泊酚对小鼠学习记忆能力的影响不具瞬时性,影响时间较长;并且未发现两药的影响程度与剂量具有明显梯度关系.%Objective: To observe the effect of Midazolam and Propofol on learning memory of mice. Methods: 88 mice were divided into 8 groups, each group had 11 mice: Midazolam group (15,30.60 mg/kg, intraperitoneal injection), Propofol group(60,120,240 mg/kg, intraperitoneal injection),normal saline group(10 ml/kg, intraperitoneal injection), and blank control group. Then using self-built water maze to test the effect of medicine on leaming memory of mice. Results: The first time after medication, as soon as they backed to normal, tested them and found out the latency of all the Midazolam groups was prolonged(15 mg/kg: t=2.693 , P=0.023 ; 30 mg/kg: t=4.633 , P=0.001 ; 60 mg/kg: t=2.550 ,P=0.029).There was no difference among other groups(P＞0.05).Keeping on injecting two days,24 hours later, test them again and find out that the latency of Propofol groups(120,240 mg/kg)was reduced(120 mg/kg: t=2.447,P=0.034;240 mg/kg: t=4.619,P=0.001).There was no difference among other groups (P＞0.05). Conclusion: The effect of Midazolam is instantaneous other than persistent. While the effect of Propofol is opposite, and there is no clear relationship between the degree of influence and dose.
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