Objective: To estibalish the rats model of high-altitude pulmonary edema by putting Wistar rats in hypobaric chamber, and compare the severity of pulmonary edema in different hypoxia time. Methods: Male Wistar rats were randomly divided into three groups: control group, 24 h hypoxia group, 48 h hypoxia group. Except for the control group, rats in the other groups were put in hypobaric chamber for 24 h and 48 h to produce a rat model of high-altitude pulmonary edema (HAPE). The wet to dry ratio (W/D), lung oxide synthase (NOS), lung endothelin (ET-1) and histological measurements were conducted on each animal. Results: Compared with the control group, the W/D ratio and the ET-1 had significantly increased, the activity of NOS was lower significantly in the 24 h hypoxia group and 48 h hypoxia group, and they all had statistically significant differences (P＜0.05). The interstitial pulmonary edema performance of the alvedar interval widened and interstitial hyperemia were found in the 24 h and 48 h hypoxia group. Comparied with the 24 h hypoxia group, the wet to dry ratio and the endothelin had significantly increased, the activity of NOS were significantly lower of the 48 h hypoxia group, and there was a statistically significant difference (P＜005). Conclusion: The model of high altitude pulmonary interstitial edema is successful set up by putting the Wistar rats in hypobaric chamber for 24 h and 48 h, the severity of pulmonary edema in the 48 h hypoxia group is more serious than the 24 h hypoxia group, which is more suitable for reality research.%目的:通过模拟高原低压低氧环境,建立高原肺水肿大鼠动物模型,探讨低氧时间对大鼠肺水肿程度影响.方法:雄性Wistar大鼠随机分为3组,分别为对照组、24 h低氧组、48 h低氧组.比较大鼠肺湿干比、肺组织匀浆中一氧化氮合酶(NOS)、内皮素-1(ET-1)及肺组织病理形态学的变化.结果:与地面对照组比较,24 h低氧组与48 h低氧组大鼠肺湿干比及肺组织匀浆内ET-1升高、NOS降低,差异均有统计学意义(均P<0.05);病理学显示,24 h低氧组与48 h低氧组均有肺泡间隔增宽、间质充血等间质性肺水肿表现,与24 h低氧组相比,48 h低氧组肺湿干比、ET-1升高,肺组织匀浆内NOS降低,差异有统计学意义(P<0.05).结论:低压低氧可以有效诱导大鼠产生间质性肺水肿,且48 h低氧大鼠肺水肿程度较24 h低氧更严重,更适合应于现实研究.
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