Objective To study the clinical value of three kinds of EB virus antibody detection in screening of nasopharyngeal carcinoma.Methods 100 cases of nasopharyngeal carcinoma diagnosed by pathological diagnosis in our hospital from September 2014 to September 2015 were selected as the research objects, 500 cases of non-nasopharyngeal carcinoma patients with nasopharyngeal symptoms and 1000 cases of healthy people in the same period were selected as control. Enzyme linked immunosorbent assay (ELISA) were used to respectively test the three groups of subjects three EB virus antibody. The positive detection rate among the three groups was compared, and the sensitivity, specificity, accuracy, negative prediction value and positive predictive value of different EB virus antibody alone or in combination with diagnosis of nasopharyngeal carcinoma(NPC) were calculated.ResultsThe comparison of VCA IgA, EA and RTA IgG detection positive rate of the three groups was patients with nasopharyngeal carcinoma>patients in control group I>Normal persons in control group II. And the detection results of patients with nasopharyngeal carcinoma group and the other two groups were statistically significant difference(P<0.05).The detection results of different EB virus were the following, the VCA-IgA and Rta-IgG of nasopharyngeal carcinoma group were significantly higher than the EA-IgA, while the EA-IgA of control group was significantly higher than the EA-IgA and Rta-IgG, the difference was significant. Three kinds of EB virus in nasopharyngeal cancer diagnosis sensitivity, specificity, accuracy, negative predicted value, and positive predictive value comparison were combined diagnosis results, and were all higher than those of each kind of Epstein Barr virus alone diagnosis results, in addition to the positive predictive value was slightly lower (but the promotion effect of combined diagnosis is the most remarkable), all of which reached more than 95%.Conclusion The serological diagnosis of three kinds of EB virus antibody is beneficial to the effective screening of nasopharyngeal carcinoma, and it has great clinical application value.%目的：研究探讨三种EB病毒抗体检测在鼻咽癌筛查中的临床价值。方法抽取2014年9月～2015年9月我院耳鼻喉科收集的经病理诊断确诊为鼻咽癌的患者100例作为研究对象，并以同期合并鼻咽部症状到我院就诊的非鼻咽癌患者500例、接受健康体检的正常人1000例作为对照，采用酶联免疫吸附法分别对三组受检者的三种EB病毒抗体进行测定，比较三组受检者的检出阳性率，同时对不同EB病毒抗体单独或联合诊断鼻咽癌的灵敏度、特异性、准确度、阴性预测值、阳性预测值等进行计算。结果三组不同受检者的VCA-IgA、EA-IgA以及Rta-IgG检测阳性率比较有鼻咽癌组患者＞对照Ⅰ组患者＞对照Ⅱ组正常人的情况，且鼻咽癌组患者各项指标的检测结果与其他两组对照组的比较差异有统计学意义（P＜0.05）。不同EB病毒的检测结果比较，则有鼻咽癌组VCA-IgA和Rta-IgG显著高于EA-IgA，而对照组受检者VCA-IgAG显著高于EA-IgA和Rta-IgG的情况，比较差异显著。三种EB病毒诊断鼻咽癌的灵敏度、特异性、准确度、阴性预测值、阳性预测值比较均有联合诊断结果均高于每种EB病毒单独诊断时的结果，除阳性预测值略低外（但联合诊断的提升效果最为显著），均达到95%以上。结论联合三种EB病毒抗体的血清学诊断有利于鼻咽癌的有效筛查，颇具临床应用价值。
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