目的 探讨选择性5-羟色胺(5-HT)再摄取抑制药类效应中成药对心肌梗死合并抑郁大鼠5-HT系统的调节作用.方法 将30只Sprague Dawley大鼠完全随机分为对照组、西药组和中药组,各10只.对照组给予2 ml 0.9％氯化钠注射液灌胃,西药组给予舍曲林20 mg/kg与2ml 0.9％氯化钠注射液配成混悬液灌胃,中药组给予心灵丸40 mg/kg与2ml 0.9％氯化钠注射液配成混悬液灌胃,3组均药物预处理4周后,建立心肌梗死合并抑郁模型.3组成模数量及成模率均为8只(80.0％),造模成功3d后处死.检测血清5-HT水平及血小板的5-HT、5-HT2A受体(5-HT2AR)、5-HT转运体(SERT)水平.结果 西药组和中药组血清5-HT水平明显高于对照组[(373±52)、(442±23) ng/L比(182±10) ng/L];中药组血清5-HT水平明显高于西药组,差异均有统计学意义(均P ＜0.05).西药组和中药组血小板5-HT和5-HT2AR水平明显高于对照组[(221±22)、(265±29) ng/L比(181±11)ng/L;(231 ±13)、(281±49) ng/L比(206±15) ng/L],血小板SERT水平明显低于对照组[(180 ±5)、(180±7)ng/L比(282±22) ng/L];中药组血小板5-HT和5-HT2AR水平明显高于西药组,差异均有统计学意义(均P ＜0.05).结论 中药组心灵丸与西药组舍曲林在治疗心肌梗死合并抑郁的机制上有相似的双心效应.%Objective To explore the effect of Chinese patent medicine with similar effect as selective 5-hydroxytryptamine (5-HT) reuptake inhibitor on 5-HT system in rats with myocardial infarction and depression.Methods Totally 30 Sprague Dawley rats were randomly divided into control group,western medicine group and Chinese medicine group,with 10 rats in each group.The control group had 2 ml 0.9％ sodium chloride by gavage;the western medicine group had 20 mg/kg sertraline + 2 ml 0.9％ sodium chloride;the Chinese medicine group had 40 mg/kg Xinling pills + 2 ml 0.9％ sodium chloride.Three groups were treated for 3 weeks to make myocardial infarction with depression models;the molding rates were 80.0％ (8/10) in all groups;the rats were executed 3 d after molding.Serum level of 5-HT and levels of 5-HT,5-HT2A receptor(5-HT2AR) and 5-HT transporter(SERT) in platelet were detected.Results Serum levels of 5-HT in western medicine group and Chinese medicine group were significantly higher than the serum level of 5-HT in control group [(373 ± 52),(442 ± 23)ng/L vs (182 ± 10)ng/L] (P ＜ 0.05);the serum level of 5-HT in Chinese medicine group was significantly higher than that in western medicine group(P ＜ 0.05).Levels of 5-HT and 5-HT2AR in platelet in western medicine group and Chinese medicine group were significantly higher and the level of SERT in platelet was significantly lower than those in control group [(221 ± 22),(265 ± 29) ng/L vs (181 ± 11) ng/L;(231 ±13),(281 ±49)ng/L vs (206±15)ng/L;(180 ±5),(180±7)ng/L vs (282 ±22)ng/L] (P＜0.05);levels of 5-HT and 5-HT2AR in platelet in Chinese medicine group were significantly higher than those in western medicine group (P ＜ 0.05).Conclusion Chinese medicine Xinling pills and western medicine sertraline have similar action in treating myocardial infarction complicated with depression in rats therapeutic.
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