目的：探讨核转录因子kappaB p65(NF-κB p65)在不同部位子宫内膜异位病灶中的表达及意义。方法收集我院住院手术的子宫内膜异位症手术组织标本48例(包括盆腔外特殊部位的子宫内膜异位症16例，卵巢子宫内膜异位症32例)，同期在位内膜22例，正常子宫内膜20例作为对照，采用免疫组化法检测NF-κB p65在上述各组内膜中的表达情况及与临床分期的关系。结果各组内膜均存在NF-κB p65的阳性表达，NF-κB p65蛋白表达于腺上皮细胞的胞核和胞浆，在盆腔外子宫内膜异位症异位病灶、卵巢子宫内膜异位症的异位内膜、在位内膜、正常子宫内膜中表达依次减少，差异有统计学意义(P<0.05)，增生期与分泌期的子宫内膜相比，差异无统计学意义(P>0.05)。卵巢内异症异位内膜中NF-κB p65的表达与临床分期无明显相关性。结论盆腔外子宫内膜异位症异位病灶、卵巢子宫内膜异位症的异位内膜中均存在NF-κB p65的高表达，提示其可能参与了子宫内膜异位症的发生发展过程。%Objective To explore the expressions and significance of NF-κB p65 in different place endometriosis.Methods A total of 48 specimans with endometriosis (16 cases of extrapelvic endometriosis,32 cases of ovarian en-dometriosis and meanwhile 22 cases of eutopic endometrium) were enrolled in the study,20 cases of normal en-dometrium were involed as control group. Immunoh istochemistry was used to detect the expressions of NF-κB p65 in above groups. Results The expressions of NF-κB p65 were found in the glandular epithelial cells in all groups. The ex-trapelvic endometriosis cases showed the highest expression,followed by ectopic endometrium, eutopic endometrium,and showing the lowest in normal endometrium,the difference among those groups was significant (P<0.05). However,the difference between prolificative phase and secretory phase was not significant (P>0.05). Furthermore, there was no correlation with clinical stages in ovarian endometriosis. Conclusion NF-κB p65 proteins are highly expressed in extrapelvic and ectopic endometrium,which suggests that they may participate in the development and progression of endometriosis.
展开▼
