Objective To study the role of Wnt/β-catenin signaling pathway in the trichostatin A ( TSA ) induced HepG2 cells apopto-sis. Methods HepG2 cells in logarithmic growth phase were taken and established the different drug concentrations of trichostatin in-duction groups, at the same time the control group was set up. The effect of TSA on the cell proliferation was detected by using the cell counting kit-8 ( CCK-8 ); the cell cycle and apoptosis were analyzed by using the flow cytometry; the expression of β-catenin was tested by using Western blot. Results The CCK-8 detection results showed that TSA had the proliferation inhibiting effect on the liver cancer HepG2 cells in a concentration and dose dependent manner;the cell cycle by the the flow cytometry showed that the quantity of G0/G1 phase cells in the TSA group was significantly increased ( P<0. 05 ) , while the quantity of the G2+M and S phase cells was significantly decreased ( P<0. 05 ) , indicating that TSA could arrest the cell cycle of HepG2 cells at G0/G1 phase;the apoptosis results showed that the apoptotic rate was ( 3. 82 ± 0. 23 )% in the control group, ( 23. 00 ± 0. 65 )% in the 250 nmol/L TSA group and ( 37. 85 ± 0. 87 )% in the 500 nmol/L TSA group, indicating that TSA had the role for promoting the apoptosis of HepG2 cells in a concentration dependent manner; the Western blot detection results showed that the β-catenin expression level was significantly in-creased. Conclusion TSA has the role for inducing apoptosis in HepG2 cells and the Wnt/β-catenin signaling pathway plays an im-portant role in this process.%目的：研究Wnt/β－catenin信号通路在曲古抑菌素（TSA）诱导HepG2细胞凋亡中的作用。方法取对数生长期HepG2细胞，设不同质量浓度药物诱导组，同时设空白对照组，用CCK－8法检测其对细胞增殖的影响；用流式细胞术检测细胞周期及细胞凋亡；Western Bolt法检测HepG2细胞β－catenin的表达。结果 CCK－8法检测结果显示，曲古抑菌素对肝癌HepG2有明显的增殖抑制作用并呈浓度和剂量依耐性；流式细胞术细胞周期检测结果显示，药物组G0/G1期细胞数量明显增多（ P<0.05），( G2+M )和S期细胞数量明显减少（ P<0.05），提示曲古抑菌素可将HepG2细胞周期阻滞在G0/G1期；细胞凋亡检测结果显示，空白对照组凋亡率为（3.82±0.23）%，250 nmol/L 曲古抑菌素组凋亡率为（23.00±0.65）%，500 nmol/L 曲古抑菌素组凋亡率为（37.85±0.87）%，提示 TSA 具有促进HepG2细胞凋亡的作用，且呈浓度依赖性；Western Bolt检测结果显示β－catenin表达水平均明显升高。结论曲古抑菌素有诱导HepG2细胞凋亡的作用，Wnt/β－catenin信号通路在此过程中起重要作用。
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