OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid,paeoniflorin,ferulic ac-id,berberine hydrochloride and baicalin in Lianqiao baidu pill. METHODS:HPLC was performed on the column of Agilent Eclipse XDB-C18 with mobile phase of 0.5% phosphoric acid(triethylamine adjusted to pH 3.0)-methanol(gradient elution)at a flow rate of 1.0 ml/min,the column temperature was 30 ℃,the detection wavelength was 230 nm(for paeoniflorin and berberine hydrochlo-ride),324 nm(for chlorogenic acid and ferulic acid)and 278 nm(for baicalin),and the injection volume was 5 μl. RESULTS:The linear range was 1.884-30.144 µg/ml for chlorogenic acid(r=0.999 9),0.392-6.272 µg/ml for paeoniflorin(r=0.999 7), 0.102-1.632 µg/ml for ferulic acid(r=0.999 7),1.326-21.216 µg/ml for berberine hydrochloride(r=0.999 8) and 1.95-31.2 µg/ml for baicalin(r=0.999 9),respectively;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 99.58%-102.47%(RSD=0.93%,n=9),99.21%-102.18%(RSD=0.90%,n=9),98.28%-101.23%(RSD=0.86%,n=9), 99.66%-101.84%(RSD=0.82%,n=9)and 99.18%-101.05%(RSD=0.62%,n=9). CONCLUSIONS:The method is accurate,re-liable,simple and fast,and can be used for the simultaneous determination of chlorogenic acid,paeoniflorin,ferulic acid,berber-ine hydrochloride and baicalin Lianqiao baidu pill.%目的：建立同时测定连翘败毒丸中绿原酸、芍药苷、阿魏酸、盐酸小檗碱、黄芩苷含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent Eclipse XDB-C18，流动相为0.5％磷酸（加三乙胺调pH至3.0）-甲醇（梯度洗脱），流速为1.0 ml/min，柱温为30℃，检测波长分别为230 nm（芍药苷和盐酸小檗碱）、324 nm（绿原酸和阿魏酸）和278 nm（黄芩苷），进样量为5μl。结果：绿原酸、芍药苷、阿魏酸、盐酸小檗碱和黄芩苷检测质量浓度线性范围分别为1.884～30.144、0.392～6.272、0.102～1.632、1.326～21.216、1.95～31.2µg/ml（r＝0.9999、0.9997、0.9997、0.9998、0.9999）；精密度、稳定性、重复性试验的RSD＜2％；加样回收率分别为99.58％～102.47％（RSD＝0.93％，n＝9）、99.21％～102.18％（RSD＝0.90％，n＝9）、98.28％～101.23％（RSD＝0.86％，n＝9）、99.66％～101.84％（RSD＝0.82％，n＝9）、99.18％～101.05％（RSD＝0.62％，n＝9）。结论：该方法准确可靠、操作简便快速，可用于连翘败毒丸中绿原酸、芍药苷、阿魏酸、盐酸小檗碱、黄芩苷含量的同时测定。
展开▼
