OBJECTIVE:To establish a method for the content determination of glabridin in Clycyrrhizae Radix et Rhizoma from different regions. METHODS:HPLC was performed on the column of Inertsil ODS-SP with mobile phase of acetoni-trile-0.05%phosphoric acid(gradient elution)at a flow rate of 0.8 ml/min,detection wavelength was 280 nm,the column tempera-ture was 35 ℃,and the injection volume was 20 μl. RESULTS:The linear range of glabridin was 0.906-18.12 μg/ml(r=0.999 7), RSDs of precision,stability and reproducibility tests were lower than 3%;recovery was 98.73%-101.90%(RSD=1.25%,n=6). There were obvious differences among the glabridin contents in G. uralensis from different regions. CONCLUSIONS:The method is simple and accurate with high precision,and can be used for the content determination of glabridin in Clycyrrhizae Radix et Rhi-zoma.%目的：建立测定甘草中光甘草定含量的方法，并对不同产地甘草中光甘草定的含量进行测定。方法：采用高效液相色谱法。色谱柱为Inertsil ODS，流动相为乙腈-0.05％磷酸（梯度洗脱），流速为0.8 ml/min，检测波长为280 nm，柱温为35℃，进样量为20μl。结果：光甘草定的检测进样量线性范围为0.906～18.12μg/ml（r＝0.9997）；精密度、稳定性、重复性试验的RSD＜3％；加样回收率为98.73％～101.90％，RSD＝1.25％（n＝6）。不同产地甘草中光甘草定的含量差异明显。结论：该方法操作简单、精密度高、结果准确，可用于甘草中光甘草定的含量测定。
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