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首页> 外文期刊>Phytochemical Analysis >A Simple Semi-preparative Reversed-phase HPLC/PDA Method for Separation and Quantification of Glycyrrhizin in Nine Samples of Glycyrrhiza glabra Root Collected from Different Geographical Origins
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A Simple Semi-preparative Reversed-phase HPLC/PDA Method for Separation and Quantification of Glycyrrhizin in Nine Samples of Glycyrrhiza glabra Root Collected from Different Geographical Origins

机译：一种简单的半制备型反相HPLC / PDA方法分离和定量测定来自不同地理来源的9个甘草根中甘草甜素的含量

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Introduction - Glycyrrhiza glabra L. (Fabaceae), commonly known as 'liquorice', is one of the most popular ingredients in several traditional herbal medicinal preparations, and glycyrrhizin is the major glycoside present in this plant. The content of glycyrrhizin may vary among G. glabra samples collected from various geographical origins, which may affect the therapeutic efficacy. Thus, quantification of glycyrrhizin in G. glabra samples is important. Objective - To develop and validate a simple semi-preparative reversed-phase HPLC with photodiode array (PDA) method for separation and quantification of glycyrrhizin in nine samples of G. glabra root collected from various geographical origins. Methods - Dried and ground root of G. glabra was Soxhlet-extracted sequentially with n-hexane and methanol (MeOH). The separation and quantification of glycyrrhizin was achieved on a C-18 reversed-phase semi-preparative column using a gradient mobile phase, 30-100% solvent B in solvent A in 30 min (solvent A: 0.1% v/v trifluoroacetic acid (TFA) in water and solvent B: 0.1% v/v of TFA in MeOH), at a flow rate of 3.00 mL/min and UV detection at 254 nm. Results - A simple semi-preparative reversed-phase HPLC/PDA method allowing clear separation and quantification of glycyrrhizin content in nine samples has been validated in terms of linearity, selectivity, limits of detection, precision, accuracy and detection. Concentration levels of glycyrrhizin were between 0.177 and 0.688% w/w of dry materials. Conclusion - This method is precise, less time consuming and more cost effective, and can be used for the quality control of any G. glabra sample with regard to its glycyrrhizin contents
机译：简介-甘草（Fabaceae），俗称“甘草”，是几种传统草药制剂中最受欢迎的成分之一，而甘草甜素是该植物中存在的主要糖苷。从不同地理来源收集的G. glabra样品中甘草甜素的含量可能会有所不同，这可能会影响治疗效果。因此，对G. glabra样品中的甘草甜素进行定量很重要。目的-建立并验证一种简单的半制备反相HPLC光电二极管阵列（PDA）方法，用于分离和定量测定从不同地理来源收集的9个glagla根样品中的甘草甜素。方法-用正己烷和甲醇（MeOH）依次提取索氏提取的干燥和磨碎的山茱G根。使用梯度流动相，30-100％溶剂B在溶剂A中的溶液在30分钟内在C-18反相半制备色谱柱上对甘草甜素进行分离和定量分析（溶剂A：0.1％v / v三氟乙酸（在水和溶剂B中的TFA）：0.1％v / v的TFA在MeOH中的溶液），流速为3.00 mL / min，在254 nm处进行UV检测。结果-在线性，选择性，检出限，精密度，准确度和检测方面，已经验证了一种简单的半制备型反相HPLC / PDA方法，可以清楚地分离和定量9个样品中的甘草甜素含量。甘草甜素的浓度为干物质的0.177％至0.688％w / w。结论-该方法精确，耗时少且更具成本效益，就甘草酸含量而言，可用于任何G. glabra样品的质量控制。

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