OBJECTIVE:To study the effects of Coriolus versicolor polysaccharides(CVP)on in vitro proliferation and apop-tosis of murine melanoma B16 cell and its mechanism. METHODS:MTT assay was used to determine the survival rate of murine melanoma B16 cell after cultured with DMSO (negative control),0 (blank control),0.156,0.312 5,0.625,1.25,2.5,5.0 and 10.0μg/ml CVP for 48,72 h,and the IC50 was calculated. Flow cytometry was used to determine the apoptotic rate of murine mela-noma B16 cell after cultured with DMSO(negative control),0(blank control),0.5μg/ml CVP for 24,48,72 h. Quantitation Re-al-time PCR(qRT-PCR)was used to determine the mRNA expression of cell apoptosis-related gene P53,Bcl-2 and Fas in murine melanoma B16 cell after cultured with DMSO(negative control),0.5 μg/ml CVP for 48,72 h. RESULTS:0.156-10.0 μg/ml CVP could inhibit the growth of B16 cells,in concentration and time-dependent manner within 0.156-0.625 μg/ml;IC50 of B16 cells af-ter cultured for 48 and 72 h were(0.32±0.01),(0.18±0.04)µg/ml. After cultured with 0.5μg/ml CVP for 24,48,72 h,apoptot-ic rates of B16 cells were increased significantly,compared to negative control and blank control (P<0.01). After cultured with 0.5 μg/ml CVP for 48,72 h,mRNA expression of P53,Bcl-2 and Fas were decreased significantly,compared to negative control (P<0.01). CONCLUSIONS:CVP can inhibit the proliferation and induce the apoptosis of B16 cells,the mechanism of which may be associated with the expression down-regulation of P53,Bcl-2 and Fas gene.%目的：研究云芝多糖（CVP）对小鼠黑色素瘤B16细胞体外增殖、凋亡的影响及其机制。方法：采用MTT法测定以二甲基亚砜（DMSO，阴性对照）和0（空白对照）、0.156、0.3125、0.625、1.25、2.5、5.0、10.0μg/ml CVP分别培养细胞48、72 h后的细胞存活率，计算半数抑制浓度（IC50）；流式细胞术测定以DMSO（阴性对照）和0（空白对照）、0.5μg/ml CVP分别培养细胞24、48、72 h后的细胞凋亡率；实时荧光定量聚合酶链式反应法测定以DMSO（阴性对照）、0.5μg/ml CVP培养细胞48、72 h后细胞中凋亡相关基因P53、Bcl-2、Fas mRNA的表达。结果：0.156～10.0μg/ml CVP对B16细胞的生长均有抑制作用，并在0.156～0.625μg/ml范围内呈浓度和时间依赖性，培养48、72 h的IC50分别为（0.32±0.01）、（0.18±0.04）µg/ml。0.5μg/ml CVP培养细胞24、48、72 h后，细胞凋亡率较阴性对照和空白对照均明显升高（P＜0.01）；0.5μg/ml CVP培养细胞48、72 h后，细胞中P53、Bcl-2、Fas mRNA表达水平较阴性对照明显降低（P＜0.01）。结论：CVP可抑制小鼠B16细胞的增殖并诱导其凋亡，其机制可能与下调细胞中P53、Bcl-2和Fas基因的表达有关。
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